AIMS: To study the recovery of platelet function after discontinuation of clopidogrel treatment in healthy volunteers. METHODS: Ten healthy volunteers were treated with clopidogrel (75 mg day(-1)) for 7 days. CD62P expression and PAC-1 binding were measured by flow cytometry. RESULTS: Adenosine diphosphate (ADP, 30 microM)-induced platelet responses were almost completely inhibited by clopidogrel. After discontinuation of the drug, platelet function gradually increased and complete recovery was seen 7 days after the last clopidogrel dose. The mean difference (95% CI) for ADP-induced PAC-1 binding (fluorescence intensity) between baseline and 7 days after the last dose was 0.01 (0.61, -0.59). Single cell analysis provides direct evidence for an irreversible mode of action of clopidogrel. CONCLUSIONS: This is the first report to directly demonstrate irreversibility of clopidogrel action in humans.
AIMS: To study the recovery of platelet function after discontinuation of clopidogrel treatment in healthy volunteers. METHODS: Ten healthy volunteers were treated with clopidogrel (75 mg day(-1)) for 7 days. CD62P expression and PAC-1 binding were measured by flow cytometry. RESULTS:Adenosine diphosphate (ADP, 30 microM)-induced platelet responses were almost completely inhibited by clopidogrel. After discontinuation of the drug, platelet function gradually increased and complete recovery was seen 7 days after the last clopidogrel dose. The mean difference (95% CI) for ADP-induced PAC-1 binding (fluorescence intensity) between baseline and 7 days after the last dose was 0.01 (0.61, -0.59). Single cell analysis provides direct evidence for an irreversible mode of action of clopidogrel. CONCLUSIONS: This is the first report to directly demonstrate irreversibility of clopidogrel action in humans.
Authors: P Savi; J M Pereillo; M F Uzabiaga; J Combalbert; C Picard; J P Maffrand; M Pascal; J M Herbert Journal: Thromb Haemost Date: 2000-11 Impact factor: 5.249
Authors: G Schmitz; G Rothe; A Ruf; S Barlage; D Tschöpe; K J Clemetson; A H Goodall; A D Michelson; A T Nurden; T V Shankey Journal: Thromb Haemost Date: 1998-05 Impact factor: 5.249
Authors: J Geiger; J Brich; P Hönig-Liedl; M Eigenthaler; P Schanzenbächer; J M Herbert; U Walter Journal: Arterioscler Thromb Vasc Biol Date: 1999-08 Impact factor: 8.311