Literature DB >> 11557017

A high-throughput screen for MscL channel activity and mutational phenotyping.

J A Maurer1, D A Dougherty.   

Abstract

A novel fluorescence-based screen for bacterial mechanosensitive ion-channel activity has been developed. This assay is capable of clearly distinguishing the previously observed gain of function and loss of function phenotypes for the Escherichia coli mechanosensitive channel of large conductance (Ec-MscL). The method modifies Molecular Probes' Live/Dead BacLight bacterial viability assay to monitor MscL channel activity as a function of bacterial survival from osmotic downshock.

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Year:  2001        PMID： 11557017     DOI： 10.1016/s0005-2736(01)00390-x

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  21 in total

Review 1.  The MscS and MscL families of mechanosensitive channels act as microbial emergency release valves.

Authors:  Ian R Booth; Paul Blount
Journal:  J Bacteriol       Date:  2012-06-08       Impact factor: 3.490

2.  Functional similarities between heterogeneously and homogenously expressed MscL constructs.

Authors:  Gamma Chi; Paul R Rohde; Pietro Ridone; Ben Hankamer; Boris Martinac; Michael J Landsberg
Journal:  Eur Biophys J       Date:  2015-08-02       Impact factor: 1.733

3.  Mechanosensitive channel gating transitions resolved by functional changes upon pore modification.

Authors:  Jessica L Bartlett; Yuezhou Li; Paul Blount
Journal:  Biophys J       Date:  2006-08-25       Impact factor: 4.033

4.  Mechanosensitive membrane channels in action.

Authors:  Serge Yefimov; Erik van der Giessen; Patrick R Onck; Siewert J Marrink
Journal:  Biophys J       Date:  2008-01-11       Impact factor: 4.033

5.  Conserved motifs in mechanosensitive channels MscL and MscS.

Authors:  Daniel Balleza; Froylan Gómez-Lagunas
Journal:  Eur Biophys J       Date:  2009-05-08       Impact factor: 1.733

6.  Defining the role of the tension sensor in the mechanosensitive channel of small conductance.

Authors:  Hannah R Malcolm; Yoon-Young Heo; Donald E Elmore; Joshua A Maurer
Journal:  Biophys J       Date:  2011-07-20       Impact factor: 4.033

Review 7.  Sensing and responding to membrane tension: the bacterial MscL channel as a model system.

Authors:  Irene Iscla; Paul Blount
Journal:  Biophys J       Date:  2012-07-17       Impact factor: 4.033

Review 8.  The evolutionary 'tinkering' of MscS-like channels: generation of structural and functional diversity.

Authors:  C D Cox; Y Nakayama; T Nomura; B Martinac
Journal:  Pflugers Arch       Date:  2014-05-13       Impact factor: 3.657

Review 9.  Searching for interesting channels: pairing selection and molecular evolution methods to study ion channel structure and function.

Authors:  Daniel L Minor
Journal:  Mol Biosyst       Date:  2009-06-19

10.  Cysteine scanning of MscL transmembrane domains reveals residues critical for mechanosensitive channel gating.

Authors:  Gal Levin; Paul Blount
Journal:  Biophys J       Date:  2004-05       Impact factor: 4.033
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