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Literature DB >> 11544242

Residue aspartate-147 from the third transmembrane region of Na(+)/H(+) antiporter NhaB of Vibrio alginolyticus plays a role in its activity.

T Nakamura¹, Y Fujisaki, H Enomoto, Y Nakayama, T Takabe, N Yamaguchi, N Uozumi.

Abstract

NhaB is a bacterial Na(+)/H(+) antiporter with unique topology. The pH dependence of NhaB from Vibrio alginolyticus differs from that of the Escherichia coli NhaB homolog. Replacement of Asp-147 with Glu made high H(+) concentrations a requirement for the NhaB activity. Replacement of Asp-147 with neutral amino acids inactivated NhaB.

Entities: Chemical Mutation Species

Mesh：

Substances：

Year: 2001 PMID： 11544242 PMCID： PMC95471 DOI： 10.1128/JB.183.19.5762-5767.2001

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

21 in total

1. Cloning, sequencing, and expression of the nhaB gene, encoding a Na+/H+ antiporter in Escherichia coli.

Authors: E Pinner; E Padan; S Schuldiner
Journal: J Biol Chem Date: 1992-06-05 Impact factor: 5.157

2. Ion extrusion systems in Escherichia coli.

Authors: B P Rosen
Journal: Methods Enzymol Date: 1986 Impact factor: 1.600

3. pHG165: a pBR322 copy number derivative of pUC8 for cloning and expression.

Authors: G S Stewart; S Lubinsky-Mink; C G Jackson; A Cassel; J Kuhn
Journal: Plasmid Date: 1986-05 Impact factor: 3.466

4. Proton-sodium stoichiometry of NhaA, an electrogenic antiporter from Escherichia coli.

Authors: D Taglicht; E Padan; S Schuldiner
Journal: J Biol Chem Date: 1993-03-15 Impact factor: 5.157

5. Kinetic properties of NhaB, a Na+/H+ antiporter from Escherichia coli.

Authors: E Pinner; E Padan; S Schuldiner
Journal: J Biol Chem Date: 1994-10-21 Impact factor: 5.157

6. Genetic evidence for two sequentially occupied K+ binding sites in the Kdp transport ATPase.

Authors: E T Buurman; K T Kim; W Epstein
Journal: J Biol Chem Date: 1995-03-24 Impact factor: 5.157

7. Cloning and characterization of a putative Ca2+/H+ antiporter gene from Escherichia coli upon functional complementation of Na+/H+ antiporter-deficient strains by the overexpressed gene.

Authors: D M Ivey; A A Guffanti; J Zemsky; E Pinner; R Karpel; E Padan; S Schuldiner; T A Krulwich
Journal: J Biol Chem Date: 1993-05-25 Impact factor: 5.157

8. Energy transduction in the thermophilic anaerobic bacterium Clostridium fervidus is exclusively coupled to sodium ions.

Authors: G Speelmans; B Poolman; T Abee; W N Konings
Journal: Proc Natl Acad Sci U S A Date: 1993-09-01 Impact factor: 11.205

9. Cloning and sequencing of an Na+/H+ antiporter gene from the marine bacterium Vibrio alginolyticus.

Authors: T Nakamura; Y Komano; E Itaya; K Tsukamoto; T Tsuchiya; T Unemoto
Journal: Biochim Biophys Acta Date: 1994-03-23

10. Physiological role of the chaA gene in sodium and calcium circulations at a high pH in Escherichia coli.

Authors: T Ohyama; K Igarashi; H Kobayashi
Journal: J Bacteriol Date: 1994-07 Impact factor: 3.490

7 in total

1. Na+/H+ antiport is essential for Yersinia pestis virulence.

Authors: Yusuke Minato; Amit Ghosh; Wyatt J Faulkner; Erin J Lind; Sara Schesser Bartra; Gregory V Plano; Clayton O Jarrett; B Joseph Hinnebusch; Judith Winogrodzki; Pavel Dibrov; Claudia C Häse
Journal: Infect Immun Date: 2013-06-17 Impact factor: 3.441

Residue aspartate-147 from the third transmembrane region of Na(+)/H(+) antiporter NhaB of Vibrio alginolyticus plays a role in its activity.

1. Cloning, sequencing, and expression of the nhaB gene, encoding a Na+/H+ antiporter in Escherichia coli.

2. Ion extrusion systems in Escherichia coli.

3. pHG165: a pBR322 copy number derivative of pUC8 for cloning and expression.

4. Proton-sodium stoichiometry of NhaA, an electrogenic antiporter from Escherichia coli.

5. Kinetic properties of NhaB, a Na+/H+ antiporter from Escherichia coli.

6. Genetic evidence for two sequentially occupied K+ binding sites in the Kdp transport ATPase.

7. Cloning and characterization of a putative Ca2+/H+ antiporter gene from Escherichia coli upon functional complementation of Na+/H+ antiporter-deficient strains by the overexpressed gene.

8. Energy transduction in the thermophilic anaerobic bacterium Clostridium fervidus is exclusively coupled to sodium ions.

9. Cloning and sequencing of an Na+/H+ antiporter gene from the marine bacterium Vibrio alginolyticus.

10. Physiological role of the chaA gene in sodium and calcium circulations at a high pH in Escherichia coli.

1. Na+/H+ antiport is essential for Yersinia pestis virulence.

2. Roles of NhaA, NhaB, and NhaD Na+/H+ antiporters in survival of Vibrio cholerae in a saline environment.

3. Competition is the basis of the transport mechanism of the NhaB Na+/H+ exchanger from Klebsiella pneumoniae.

4. Energetic evolution of cellular Transportomes.

Review 5. Prokaryotic Na⁺/H⁺ Exchangers-Transport Mechanism and Essential Residues.

6. Mutation of two key aspartate residues alters stoichiometry of the NhaB Na⁺/H⁺ exchanger from Klebsiella pneumoniae.

7. Comparative Genomics and Transcriptomics Analyses Reveal a Unique Environmental Adaptability of Vibrio fujianensis.

Review 5. Prokaryotic Na+/H+ Exchangers-Transport Mechanism and Essential Residues.

Review 5. Prokaryotic Na⁺/H⁺ Exchangers-Transport Mechanism and Essential Residues.