Literature DB >> 3012611

pHG165: a pBR322 copy number derivative of pUC8 for cloning and expression.

G S Stewart, S Lubinsky-Mink, C G Jackson, A Cassel, J Kuhn.   

Abstract

During the construction of the Messing pUC plasmid series, the rop(rom) gene of pBR322 which mediates the activity of RNAI was deleted. This has resulted in an elevated copy number for the pUC plasmids which makes the expression of beta-galactosidase activity constitutive in a host containing the Iqtss lac repressor. We describe the construction of a new series of vectors which retain the pUC multiple cloning site (MCS) but in which copy number control has been recovered. In addition, the lac alpha/lac promoter expression region has been inserted into a HpaI cassette. This facilitates the movement of recombinant DNA clones within the MCS. It also increases the complementation activity of the lac alpha peptide by an order of magnitude, allowing selection of recombinants by their Lac- phenotype on MacConkey agar.

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Year:  1986        PMID： 3012611     DOI： 10.1016/0147-619x(86)90035-1

Source DB:  PubMed          Journal:  Plasmid        ISSN： 0147-619X            Impact factor:   3.466


  88 in total

1.  Type III secretion chaperone-dependent regulation: activation of virulence genes by SicA and InvF in Salmonella typhimurium.

Authors:  K H Darwin; V L Miller
Journal:  EMBO J       Date:  2001-04-17       Impact factor: 11.598

2.  Identification of an ancillary protein, YabF, required for activity of the KefC glutathione-gated potassium efflux system in Escherichia coli.

Authors:  S Miller; L S Ness; C M Wood; B C Fox; I R Booth
Journal:  J Bacteriol       Date:  2000-11       Impact factor: 3.490

3.  Identification of a locus involved in systemic dissemination of Yersinia enterocolitica.

Authors:  K M Nelson; G M Young; V L Miller
Journal:  Infect Immun       Date:  2001-10       Impact factor: 3.441

4.  Mutational analysis of a conserved signal-transducing element: the HAMP linker of the Escherichia coli nitrate sensor NarX.

Authors:  J Alex Appleman; Valley Stewart
Journal:  J Bacteriol       Date:  2003-01       Impact factor: 3.490

5.  YmoA negatively regulates expression of invasin from Yersinia enterocolitica.

Authors:  Damon W Ellison; Briana Young; Kristin Nelson; Virginia L Miller
Journal:  J Bacteriol       Date:  2003-12       Impact factor: 3.490

6.  Synthetic lac operator substitutions for studying the nitrate- and nitrite-responsive NarX-NarL and NarQ-NarP two-component regulatory systems of Escherichia coli K-12.

Authors:  Valley Stewart; Peggy J Bledsoe
Journal:  J Bacteriol       Date:  2003-04       Impact factor: 3.490

7.  In vivo requirement of integration host factor for nar (nitrate reductase) operon expression in Escherichia coli K-12.

Authors:  R S Rabin; L A Collins; V Stewart
Journal:  Proc Natl Acad Sci U S A       Date:  1992-09-15       Impact factor: 11.205

8.  Molecular cloning of the Escherichia coli B L-fucose-D-arabinose gene cluster.

Authors:  E A Elsinghorst; R P Mortlock
Journal:  J Bacteriol       Date:  1994-12       Impact factor: 3.490

9.  Identification of the pcaRKF gene cluster from Pseudomonas putida: involvement in chemotaxis, biodegradation, and transport of 4-hydroxybenzoate.

Authors:  C S Harwood; N N Nichols; M K Kim; J L Ditty; R E Parales
Journal:  J Bacteriol       Date:  1994-11       Impact factor: 3.490

10.  Epithelial cell invasion and adherence directed by the enterotoxigenic Escherichia coli tib locus is associated with a 104-kilodalton outer membrane protein.

Authors:  E A Elsinghorst; J A Weitz
Journal:  Infect Immun       Date:  1994-08       Impact factor: 3.441
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