| Literature DB >> 11537919 |
G A Nelson1, W W Schubert, G A Kazarians, G F Richards.
Abstract
A subset of the Caenorhabditis elegans nematodes flown aboard Biorack on IML-1 was analyzed for the fidelity of development and the mechanics of chromosomes at meiosis. To assess meiosis, mutant worms marked at two linked or unlinked loci were inoculated as heterozygous hermaphrodites and allowed to self fertilize. Mendelian segregation ratios and recombination frequency were measured for offspring produced at 1XG or in microgravity. To assess development, worms and embryos were fixed and stained with the DNA dye, DAPI, or antibodies specific for antigens expressed in germ cells, pharyngeal and body wall muscles, and gut cells. The distribution of cytoplasmic determinants, cell nuclei counts and positions were scored to assess symmetry relations and anatomical features.Entities:
Keywords: NASA Center JPL; NASA Discipline Number 00-00; NASA Discipline Number 45-10; NASA Discipline Radiation Health; NASA Experiment Number 284050 1/1; NASA Program Flight; NASA Program Radiation Health
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Year: 1994 PMID： 11537919 DOI： 10.1016/0273-1177(94)90405-7
Source DB: PubMed Journal: Adv Space Res ISSN： 0273-1177 Impact factor: 2.152