Literature DB >> 11532441

Carbohydrate transporters of the bacterial phosphoenolpyruvate: sugar phosphotransferase system (PTS).

C Siebold1, K Flükiger, R Beutler, B Erni.   

Abstract

The glucose transporter of Escherichia coli couples translocation with phosphorylation of glucose. The IICB(Glc) subunit spans the membrane eight times. Split, circularly permuted and cyclized forms of IICB(Glc) are described. The split variant was 30 times more active when the two proteins were encoded by a dicistronic mRNA than by two genes. The stability and activity of circularly permuted forms was improved when they were expressed as fusion proteins with alkaline phosphatase. Cyclized IICB(Glc) and IIA(Glc) were produced in vivo by RecA intein-mediated trans-splicing. Purified, cyclized IIA(Glc) and IICB(Glc) had 100% and 30% of wild-type glucose phosphotransferase activity, respectively. Cyclized IIA(Glc) displayed increased stability against temperature and GuHCl-induced unfolding.

Entities:  

Mesh：

Substances：

Year:  2001        PMID： 11532441     DOI： 10.1016/s0014-5793(01)02705-3

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  46 in total

1.  Identification of potential type III secretion proteins via heterologous expression of Vibrio parahaemolyticus DNA.

Authors:  Xiaohui Zhou; Seth D Nydam; Jeffrey E Christensen; Michael E Konkel; Lisa Orfe; Patrick Friel; Douglas R Call
Journal:  Appl Environ Microbiol       Date:  2012-03-02       Impact factor: 4.792

2.  Expression, purification, crystallization and preliminary X-ray analysis of the EIICGlc domain of the Escherichia coli glucose transporter.

Authors:  Andreas Zurbriggen; Philipp Schneider; Priska Bähler; Ulrich Baumann; Bernhard Erni
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2010-05-26

3.  Salmonella pathogenicity island 2 expression negatively controlled by EIIANtr-SsrB interaction is required for Salmonella virulence.

Authors:  Jeongjoon Choi; Dongwoo Shin; Hyunjin Yoon; Jiae Kim; Chang-Ro Lee; Minjeong Kim; Yeong-Jae Seok; Sangryeol Ryu
Journal:  Proc Natl Acad Sci U S A       Date:  2010-11-08       Impact factor: 11.205

4.  Solution structure of the IIAChitobiose-HPr complex of the N,N'-diacetylchitobiose branch of the Escherichia coli phosphotransferase system.

Authors:  Young-Sang Jung; Mengli Cai; G Marius Clore
Journal:  J Biol Chem       Date:  2012-05-16       Impact factor: 5.157

Review 5.  How phosphotransferase system-related protein phosphorylation regulates carbohydrate metabolism in bacteria.

Authors:  Josef Deutscher; Christof Francke; Pieter W Postma
Journal:  Microbiol Mol Biol Rev       Date:  2006-12       Impact factor: 11.056

6.  The mannose transporter complex: an open door for the macromolecular invasion of bacteria.

Authors:  Bernhard Erni
Journal:  J Bacteriol       Date:  2006-10       Impact factor: 3.490

7.  Intramolecular domain-domain association/dissociation and phosphoryl transfer in the mannitol transporter of Escherichia coli are not coupled.

Authors:  Jeong-Yong Suh; Junji Iwahara; G Marius Clore
Journal:  Proc Natl Acad Sci U S A       Date:  2007-02-21       Impact factor: 11.205

8.  Thermodynamic dissection of large-scale domain motions coupled with ligand binding of enzyme I.

Authors:  Young-Joo Yun; Ban-Seok Choi; Eun-Hee Kim; Jeong-Yong Suh
Journal:  Protein Sci       Date:  2013-10-09       Impact factor: 6.725

9.  Solution structure of the IIAChitobiose-IIBChitobiose complex of the N,N'-diacetylchitobiose branch of the Escherichia coli phosphotransferase system.

Authors:  Young-Sang Jung; Mengli Cai; G Marius Clore
Journal:  J Biol Chem       Date:  2009-12-03       Impact factor: 5.157

10.  Characterization of soluble enzyme II complexes of the Escherichia coli phosphotransferase system.

Authors:  Mohammad Aboulwafa; Milton H Saier
Journal:  J Bacteriol       Date:  2004-12       Impact factor: 3.490
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.