BACKGROUND: The saphenous vein is the most commonly used conduit for coronary artery bypass surgery, but 1-year occlusion rates as high as 30 per cent have been reported. In conventional surgery, considerable damage to the vein occurs during harvesting. The aim of this study was to compare endothelial integrity and nitric oxide synthase (NOS) in saphenous veins harvested by a novel 'no-touch' technique and veins harvested conventionally. METHODS: Immunohistochemistry was used to study endothelial integrity, and a combination of histochemistry and autoradiography was employed to identify NOS in human saphenous veins harvested by conventional and no-touch techniques. RESULTS: The endothelial lining of conventional grafts was reduced compared with that of no-touch grafts (52 versus 73 per cent; P = 0.04). This was associated with a concomitant reduction of NOS availability; NOS was also present in the adventitial vasa vasorum of no-touch vessels. CONCLUSION: Some of the sites with potential for nitric oxide release in vivo are removed during conventional saphenous vein harvesting. These sites were preserved after no-touch harvesting, suggesting the potential to improve coronary artery bypass graft patency.
BACKGROUND: The saphenous vein is the most commonly used conduit for coronary artery bypass surgery, but 1-year occlusion rates as high as 30 per cent have been reported. In conventional surgery, considerable damage to the vein occurs during harvesting. The aim of this study was to compare endothelial integrity and nitric oxide synthase (NOS) in saphenous veins harvested by a novel 'no-touch' technique and veins harvested conventionally. METHODS: Immunohistochemistry was used to study endothelial integrity, and a combination of histochemistry and autoradiography was employed to identify NOS in human saphenous veins harvested by conventional and no-touch techniques. RESULTS: The endothelial lining of conventional grafts was reduced compared with that of no-touch grafts (52 versus 73 per cent; P = 0.04). This was associated with a concomitant reduction of NOS availability; NOS was also present in the adventitial vasa vasorum of no-touch vessels. CONCLUSION: Some of the sites with potential for nitric oxide release in vivo are removed during conventional saphenous vein harvesting. These sites were preserved after no-touch harvesting, suggesting the potential to improve coronary artery bypass graft patency.
Authors: Bhuvaneswari Krishnamoorthy; William R Critchley; Rajamiyer V Venkateswaran; James Barnard; Ann Caress; James E Fildes; Nizar Yonan Journal: J Cardiothorac Surg Date: 2016-04-08 Impact factor: 1.637
Authors: Nikolaos A Papakonstantinou; Nikolaos G Baikoussis; John Goudevenos; George Papadopoulos; Efstratios Apostolakis Journal: Ann Card Anaesth Date: 2016 Jul-Sep
Authors: M S Fernández-Alfonso; M Gil-Ortega; I Aranguez; D Souza; M Dreifaldt; B Somoza; M R Dashwood Journal: Br J Pharmacol Date: 2017-03-02 Impact factor: 8.739
Authors: Lene P Vestergaard; Leila Benhassen; Ivy S Modrau; Frank de Paoli; Ebbe Boedtkjer Journal: Front Physiol Date: 2018-01-12 Impact factor: 4.566