Literature DB >> 11526144

Enzyme-linked immunosorbent assay for detecting herpesvirus exposure in Mediterranean tortoises (spur-thighed tortoise [Testudo graeca] and Hermann's tortoise [Testudo hermanni]).

F C Origgi1, P A Klein, K Mathes, S Blahak, R E Marschang, S J Tucker, E R Jacobson.   

Abstract

An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of antibodies to a herpesvirus associated with an upper respiratory tract disease in Mediterranean tortoises [spur-thighed tortoise (Testudo graeca) and Hermann's tortoise (Testudo hermanni)]. This serodiagnostic test was validated through a hyperimmunization study. The mean of the A(405) readings of the plasma samples collected at time zero of the hyperimmunization study plus three times the standard deviation was used as the cutoff for seropositivity in tortoises. ELISA results were compared to serum neutralization (SN) values for the same samples by using the McNemar test. The results obtained by SN and ELISA were not significantly different (P > 0.05). This new ELISA could be used as an important diagnostic tool for screening wild populations and private and zoo collections of Mediterranean tortoises.

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Year:  2001        PMID： 11526144      PMCID： PMC88312          DOI： 10.1128/JCM.39.9.3156-3163.2001

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  21 in total

1.  A herpesvirus-type agent associated with skin lesions of green sea turtles in aquaculture.

Authors:  G Rebell; A Rywlin; H Haines
Journal:  Am J Vet Res       Date:  1975-08       Impact factor: 1.156

2.  Detection of herpesvirus-like and papillomavirus-like particles associated with diseases of tortoises.

Authors:  S E Drury; R E Gough; S McArthur; M Jessop
Journal:  Vet Rec       Date:  1998-12-05       Impact factor: 2.695

3.  Herpesvirus-like infection in two Pacific pond turtles.

Authors:  F L Frye; L S Oshiro; F R Dutra; J D Carney
Journal:  J Am Vet Med Assoc       Date:  1977-11-01       Impact factor: 1.936

4.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

5.  Evolution of low molecular weight immunoglobulins. I. Relationship of 7S immunoglobulins of various vertebrates to chicken IgY.

Authors:  D Hädge; H Fiebig; H Ambrosius
Journal:  Dev Comp Immunol       Date:  1980       Impact factor: 3.636

6.  Detection of chelonid herpesvirus DNA by nonradioactive in situ hybridization in tissues from tortoises suffering from stomatitis-rhinitis complex in Europe and North America.

Authors:  J P Teifke; C V Löhr; R E Marschang; N Osterrieder; H Posthaus
Journal:  Vet Pathol       Date:  2000-09       Impact factor: 2.221

7.  Herpesvirus infection in tortoises (Malacochersus tornieri and Testudo horsfieldii).

Authors:  Y Une; K Uemura; Y Nakano; J Kamiie; T Ishibashi; Y Nomura
Journal:  Vet Pathol       Date:  1999-11       Impact factor: 2.221

8.  Antibody responses of tumor-bearing mice to their own tumors captured and perpetuated as hybridomas.

Authors:  C R Simrell; P A Klein
Journal:  J Immunol       Date:  1979-11       Impact factor: 5.422

9.  Herpesvirus-like infection in a painted turtle (Chrysemys picta).

Authors:  W R Cox; W A Rapley; I K Barker
Journal:  J Wildl Dis       Date:  1980-07       Impact factor: 1.535

10.  Phylogen of immunoglobulin structure and function. 3. Immunoglobulins of the chicken.

Authors:  G A Leslie; L W Clem
Journal:  J Exp Med       Date:  1969-12-01       Impact factor: 14.307
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  6 in total

1.  Generation of Anti-Boa Immunoglobulin Antibodies for Serodiagnostic Applications, and Their Use to Detect Anti-Reptarenavirus Antibodies in Boa Constrictor.

Authors:  Yegor Korzyukov; Udo Hetzel; Anja Kipar; Olli Vapalahti; Jussi Hepojoki
Journal:  PLoS One       Date:  2016-06-29       Impact factor: 3.240

2.  Herpesvirus associated dermal papillomatosis in Williams' mud turtle Pelusios williamsi with effects of autogenous vaccine therapy.

Authors:  Pavel Široký; Fredric L Frye; Nela Dvořáková; Martin Hostovský; Hynek Prokop; Pavel Kulich
Journal:  J Vet Med Sci       Date:  2018-06-11       Impact factor: 1.267

3.  Antibodies against Two Testudinid Herpesviruses in Pet Tortoises in Europe.

Authors:  Christoph Leineweber; Rachel E Marschang
Journal:  Animals (Basel)       Date:  2022-09-05       Impact factor: 3.231

4.  A Genomic Approach to Unravel Host-Pathogen Interaction in Chelonians: The Example of Testudinid Herpesvirus 3.

Authors:  Francesco C Origgi; Marco Tecilla; Paola Pilo; Fabio Aloisio; Patricia Otten; Lisandra Aguilar-Bultet; Ursula Sattler; Paola Roccabianca; Carlos H Romero; David C Bloom; Elliott R Jacobson
Journal:  PLoS One       Date:  2015-08-05       Impact factor: 3.240

5.  Clinical, virological and epidemiological characterization of an outbreak of Testudinid Herpesvirus 3 in a chelonian captive breeding facility: Lessons learned and first evidence of TeHV3 vertical transmission.

Authors:  Maria Luisa Marenzoni; Lorenzo Santoni; Andrea Felici; Carmen Maresca; Valentina Stefanetti; Monica Sforna; Maria Pia Franciosini; Patrizia Casagrande Proietti; Francesco Carlo Origgi
Journal:  PLoS One       Date:  2018-05-10       Impact factor: 3.240

6.  The Genome of a Tortoise Herpesvirus (Testudinid Herpesvirus 3) Has a Novel Structure and Contains a Large Region That Is Not Required for Replication In Vitro or Virulence In Vivo.

Authors:  Frédéric Gandar; Gavin S Wilkie; Derek Gatherer; Karen Kerr; Didier Marlier; Marianne Diez; Rachel E Marschang; Jan Mast; Benjamin G Dewals; Andrew J Davison; Alain F C Vanderplasschen
Journal:  J Virol       Date:  2015-09-02       Impact factor: 5.103
  6 in total

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