Literature DB >> 11526053

Rapid confirmation of Clostridium perfringens by using chromogenic and fluorogenic substrates.

P W Adcock1, C P Saint.   

Abstract

The use of 4-methylumbelliferyl phosphate (MUP) and ortho-nitrophenyl-beta-D-galactopyranoside (ONPG) for the identification of Clostridium perfringens was investigated. A liquid assay containing both MUP and ONPG was a highly specific alternative method for C. perfringens confirmation, reducing incubation time from 48 to only 4 h. The assay solution is easy to prepare, does not require anaerobic conditions for use, and has an extended shelf life.

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Year:  2001        PMID： 11526053      PMCID： PMC93177          DOI： 10.1128/AEM.67.9.4382-4384.2001

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  8 in total

1.  Benthic Distribution of Sewage Sludge Indicated by Clostridium perfringens at a Deep-Ocean Dump Site.

Authors:  R T Hill; I T Knight; M S Anikis; R R Colwell
Journal:  Appl Environ Microbiol       Date:  1993-01       Impact factor: 4.792

2.  Membrane filter enumeration method for Clostridium perfringens.

Authors:  J W Bisson; V J Cabelli
Journal:  Appl Environ Microbiol       Date:  1979-01       Impact factor: 4.792

3.  Acid phosphatase in Clostridium perfringens. A new rapid and simple identification method.

Authors:  K Ueno; H Fujii; T Marui; J Takahashi; T Sugitani
Journal:  Jpn J Microbiol       Date:  1970-03

4.  National field evaluation of a defined substrate method for the simultaneous enumeration of total coliforms and Escherichia coli from drinking water: comparison with the standard multiple tube fermentation method.

Authors:  S C Edberg; M J Allen; D B Smith
Journal:  Appl Environ Microbiol       Date:  1988-06       Impact factor: 4.792

5.  Influence of sodium chloride on the beta-glucuronidase activity of Clostridium perfringens and Escherichia coli.

Authors:  T Fujisawa; K Aikawa; T Takahashi; S Yamai
Journal:  Lett Appl Microbiol       Date:  2000-09       Impact factor: 2.858

6.  Inactivation of Cryptosporidium parvum oocysts and Clostridium perfringens spores by a mixed-oxidant disinfectant and by free chlorine.

Authors:  L V Venczel; M Arrowood; M Hurd; M D Sobsey
Journal:  Appl Environ Microbiol       Date:  1997-04       Impact factor: 4.792

7.  Clostridium perfringens and somatic coliphages as indicators of the efficiency of drinking water treatment for viruses and protozoan cysts.

Authors:  P Payment; E Franco
Journal:  Appl Environ Microbiol       Date:  1993-08       Impact factor: 4.792

8.  Rapid detection of total and fecal coliforms in water by enzymatic hydrolysis of 4-methylumbelliferone-beta-D-galactoside.

Authors:  J D Berg; L Fiksdal
Journal:  Appl Environ Microbiol       Date:  1988-08       Impact factor: 4.792
  8 in total
  7 in total

Review 1.  Methodologies for the characterization of microbes in industrial environments: a review.

Authors:  Johanna Maukonen; Jaana Mättö; Gun Wirtanen; Laura Raaska; Tiina Mattila-Sandholm; Maria Saarela
Journal:  J Ind Microbiol Biotechnol       Date:  2003-05-23       Impact factor: 3.346

Review 2.  Use of bacterial spores in monitoring water quality and treatment.

Authors:  Gerard N Stelma
Journal:  J Water Health       Date:  2018-08       Impact factor: 1.744

3.  Abilities of the mCP Agar method and CRENAME alpha toxin-specific real-time PCR assay to detect Clostridium perfringens spores in drinking water.

Authors:  Andrée F Maheux; Eve Bérubé; Dominique K Boudreau; Romain Villéger; Philippe Cantin; Maurice Boissinot; Luc Bissonnette; Michel G Bergeron
Journal:  Appl Environ Microbiol       Date:  2013-09-27       Impact factor: 4.792

4.  Characterization of a unique class C acid phosphatase from Clostridium perfringens.

Authors:  Thomas J Reilly; Deborah L Chance; Michael J Calcutt; John J Tanner; Richard L Felts; Stephen C Waller; Michael T Henzl; Thomas P Mawhinney; Irene K Ganjam; William H Fales
Journal:  Appl Environ Microbiol       Date:  2009-04-10       Impact factor: 4.792

5.  Expression, purification and crystallization of class C acid phosphatases from Francisella tularensis and Pasteurella multocida.

Authors:  Harkewal Singh; Richard L Felts; Li Ma; Thomas J Malinski; Michael J Calcutt; Thomas J Reilly; John J Tanner
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2009-02-12

6.  Acid phosphatase test proves superior to standard phenotypic identification procedure for Clostridium perfringens strains isolated from water.

Authors:  G Ryzinska-Paier; R Sommer; J M Haider; S Knetsch; C Frick; A K T Kirschner; A H Farnleitner
Journal:  J Microbiol Methods       Date:  2011-08-18       Impact factor: 2.363

Review 7.  Recent Developments of the Synthetic Biology Toolkit for Clostridium.

Authors:  Rochelle C Joseph; Nancy M Kim; Nicholas R Sandoval
Journal:  Front Microbiol       Date:  2018-02-12       Impact factor: 5.640
  7 in total

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