| Literature DB >> 11523797 |
I Mendizabal1, A Pascual-Ahuir, R Serrano, I F de Larrinoa.
Abstract
In Saccharomyces cerevisiae the transcription of the ENA1 gene is modulated by multiple transduction pathways that respond to osmotic, ionic and nutrient stresses. We have investigated the molecular mechanisms involved in ENA1 induction by the calcium-calcineurin-activated transcription factor Crzl/Tcn1. We found in the ENA1 promoter a calcium-responsive, Crzl-dependent upstream activating region (UASENA1) located between -713 bp and 826 bp relative to the translation start. This region contains two separate control elements: the upstream element (5'-GAATGGCTG-3') between -813 and -821 binds Crzlp with lower affinity and mostly contributes to basal ENA1 expression, whereas the downstream element (5'-GGGTGGCTG-3') between 727 and 719 binds Crz1p with higher affinity and is a major determinant of the induction response to calcium.Entities:
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Year: 2001 PMID： 11523797 DOI： 10.1007/s004380100474
Source DB: PubMed Journal: Mol Genet Genomics ISSN： 1617-4623 Impact factor: 3.291