Y Chen1, J Li, M Zeng, L Lu, D Qu, Y Mao, Z Fan, J Hua. 1. Shanghai Institute of Digestive Diseases, Renji Hospital, Shanghai Second Medical University, Shanghai 200001, China.
Abstract
OBJECTIVE: To study the inhibitory effect of oxymatrine on HCV in vitro. METHODS: SMMC-7721 cells transfected with pBK-HCV using lipofectin transfection protocal were treated with oxymatrine. bDNA signal amplification assay and MTT colorimetric assay were used for intracellular HCV RNA and cytotoxicity. RESULTS: The stable HCV expression cell model established here could be used as a tool for medicine screen. Oxymatrine at the concentration of 100~1000 mug/ml could significantly decrease the level of intracellular HCV RNA. No cytotoxicity was shown in such concentrations. CONCLUSIONS: Oxymatrine has an anti-HCV potential activity in vitro.
OBJECTIVE: To study the inhibitory effect of oxymatrine on HCV in vitro. METHODS:SMMC-7721 cells transfected with pBK-HCV using lipofectin transfection protocal were treated with oxymatrine. bDNA signal amplification assay and MTT colorimetric assay were used for intracellular HCV RNA and cytotoxicity. RESULTS: The stable HCV expression cell model established here could be used as a tool for medicine screen. Oxymatrine at the concentration of 100~1000 mug/ml could significantly decrease the level of intracellular HCV RNA. No cytotoxicity was shown in such concentrations. CONCLUSIONS:Oxymatrine has an anti-HCV potential activity in vitro.
Authors: Muhammad Daniyal; Muhammad Akram; Rida Zainab; Naveed Munir; Aamir Sharif; Syed Muhammad Ali Shah; Bin Liu; Wei Wang Journal: Inflammopharmacology Date: 2019-02-08 Impact factor: 4.473
Authors: A C G Jardim; Z Igloi; J F Shimizu; V A F F M Santos; L G Felippe; B F Mazzeu; Y Amako; M Furlan; M Harris; P Rahal Journal: Antiviral Res Date: 2014-12-31 Impact factor: 5.970