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Literature DB >> 11502257

Neurotrophin-induced transport of a beta-actin mRNP complex increases beta-actin levels and stimulates growth cone motility.

H L Zhang¹, T Eom, Y Oleynikov, S M Shenoy, D A Liebelt, J B Dictenberg, R H Singer, G J Bassell.

Abstract

Neurotrophin regulation of actin-dependent changes in growth cone motility may depend on the signaling of beta-actin mRNA transport. Formation of an RNP complex between the beta-actin mRNA zipcode sequence and Zipcode Binding Protein 1 (ZBP1) was required for its localization to growth cones. Antisense oligonucleotides to the zipcode inhibited formation of this RNP complex in vitro and the neurotrophin-induced localization of beta-actin mRNA and ZBP1 granules. Live cell imaging of neurons transfected with EGFP-ZBP1 revealed fast, bidirectional movements of granules in neurites that were inhibited by antisense treatment, as visualized by FRAP analysis. NT-3 stimulation of beta-actin protein localization was dependent on the 3'UTR and inhibited by antisense treatment. Growth cones exhibited impaired motility in the presense of antisense. These results suggest a novel mechanism to influence growth cone dynamics involving the regulated transport of mRNA.

Entities: Chemical Disease Gene

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Year: 2001 PMID： 11502257 DOI： 10.1016/s0896-6273(01)00357-9

Source DB: PubMed Journal: Neuron ISSN： 0896-6273 Impact factor: 17.173

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Cited

160 in total

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Authors: Huidong Wang; Anna Iacoangeli; Susanna Popp; Ilham A Muslimov; Hiroaki Imataka; Nahum Sonenberg; Ivan B Lomakin; Henri Tiedge
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4. A receptor for activated C kinase is part of messenger ribonucleoprotein complexes associated with polyA-mRNAs in neurons.

Authors: Frank Angenstein; Anne M Evans; Robert E Settlage; Stewart T Moran; Shuo-Chien Ling; Anna Y Klintsova; Jeffrey Shabanowitz; Donald F Hunt; William T Greenough
Journal: J Neurosci Date: 2002-10-15 Impact factor: 6.167

Neurotrophin-induced transport of a beta-actin mRNP complex increases beta-actin levels and stimulates growth cone motility.

1. Target interaction regulates distribution and stability of specific mRNAs.

2. Transport of Neuronal BC1 RNA in Mauthner Axons.

3. Dendritic BC1 RNA: functional role in regulation of translation initiation.

4. A receptor for activated C kinase is part of messenger ribonucleoprotein complexes associated with polyA-mRNAs in neurons.

5. The KH domains of Xenopus Vg1RBP mediate RNA binding and self-association.

6. Real-time visualization of ZBP1 association with beta-actin mRNA during transcription and localization.

7. Nuclear transit of human zipcode-binding protein IMP1.

Review 8. Imaging of single mRNAs in the cytoplasm of living cells.

Review 9. Mechanisms and consequences of subcellular RNA localization across diverse cell types.

Review 10. mRNA localization: gene expression in the spatial dimension.