An easy procedure for in vivo estimation of protein binding and correction of elevated serum values induced by venous stasis.

Venous stasis augments the apparent concentrations of serum proteins. A theoretical mathematical model for the correction of serum protein-bound constituents is propounded and an easy procedure for such corrections described. It is suggested that the effect of venous stasis can also be utilized for in vivo estimation of the average protein binding. The hypotheses were tested in 19 epileptic patients. The results obtained by serum determinations of sodium, magnesium, calcium and phenytoin indicate the validity of the theories.