Literature DB >> 11488618

Colorimetric assay method for determination of the tannin acyl hydrolase (EC 3.1.1.20) activity.

K C Mondal1, D Banerjee, M Jana, B R Pati.   

Abstract

A new colorimetric method of tannase (tannin acyl hydrolase, EC 3.1.1.20) assay has been developed using its specific substrate tannic acid. It is based on the changes in optical density of substrate tannic acid after enzymatic reaction at 530 nm. The residual tannic acid was measured by a modified BSA precipitation method. This assay is very simple, reproducible, and very convenient, and with it tannase activity can be measured in relation to the growth of the organism. Copyright 2001 Academic Press.

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Year:  2001        PMID： 11488618     DOI： 10.1006/abio.2001.5185

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  7 in total

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2.  Statistical optimization of process parameters for the production of tannase by Aspergillus flavus under submerged fermentation.

Authors:  S K Mohan; T Viruthagiri; C Arunkumar
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Journal:  Biotechnol Rep (Amst)       Date:  2015-06-26

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Journal:  Braz J Microbiol       Date:  2012-06-01       Impact factor: 2.476

7.  The Different Contributors to Antioxidant Activity in Thermally Dried Flesh and Peel of Astringent Persimmon Fruit.

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Journal:  Antioxidants (Basel)       Date:  2022-03-21
  7 in total

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