| Literature DB >> 11486830 |
A E Katrusiak1, P G Paterson, H Kamencic, A Shoker, A W Lyon.
Abstract
A sensitive high-performance liquid chromatographic method for quantification of sulphydryl and disulfide amino acids in human plasma using ultra violet spectrophotometric detection was developed. Precolumn derivatization with 5,5'-dithio-bis-nitrobenzoic acid (DTNB) and an optional pre-derivatization reaction with dithiothreitol allowed both quantitative reduction of disulfides for measurement of total amino acid levels and the measurement of the reduced forms. A dynamic range of 500 nmol/l-750 micromol/l allowed the major analytes of interest to be quantified in plasma without sample dilution. The assay is a sensitive and precise method for the determination of sulphydryl and disulfide amino acids in plasma and cell extracts.Entities:
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Year: 2001 PMID： 11486830 DOI： 10.1016/s0378-4347(01)00182-7
Source DB: PubMed Journal: J Chromatogr B Biomed Sci Appl ISSN： 1387-2273