| Literature DB >> 11469400 |
M A Statnick1, L S Beavers, L J Conner, H Corominola, D Johnson, C D Hammond, R Rafaeloff-Phail, T Seng, T M Suter, J P Sluka, E Ravussin, R A Gadski, J F Caro.
Abstract
We have screened a subtracted cDNA library in order to identify differentially expressed genes in omental adipose tissue of human patients with Type 2 diabetes. One clone (#1738) showed a marked reduction in omental adipose tissue from patients with Type 2 diabetes. Sequencing and BLAST analysis revealed clone #1738 was the adipocyte-specific secreted protein gene apM1 (synonyms ACRP30, AdipoQ, GBP28). Consistent with the murine orthologue, apM1 mRNA was expressed in cultured human adipocytes and not in preadipocytes. Using RT-PCR we confirmed that apM1 mRNA levels were significantly reduced in omental adipose tissue of obese patients with Type 2 diabetes compared with lean and obese normoglycemic subjects. Although less pronounced, apM1 mRNA levels were reduced in subcutaneous adipose tissue of Type 2 diabetic patients. Whereas the biological function of apM1 is presently unknown, the tissue specific expression, structural similarities to TNFalpha, and the dysregulated expression observed in obese Type 2 diabetic patients suggest that this factor may play a role in the pathogenesis of insulin resistance and Type 2 diabetes.Entities:
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Year: 2000 PMID： 11469400 PMCID： PMC2477762 DOI： 10.1155/edr.2000.81
Source DB: PubMed Journal: Int J Exp Diabetes Res ISSN： 1560-4284