BACKGROUND: We used a combination of genetic subtraction, silicon DNA microarray analysis, and quantitative PCR to identify tissue- and tumor-specific genes as diagnostic targets for breast cancer. METHODS AND RESULTS: From a large number of candidate antigens, several specific subsets of genes were identified that showed concordant and complementary expression profiles. Whereas transcriptional profiling of mammaglobin resulted in the detection of 70% of tumors in a panel of 46 primary and metastatic breast cancers, the inclusion of three additional markers resulted in detection of all 46 specimens. Immunomagnetic epithelial cell enrichment of circulating tumor cells from the peripheral blood of patients with metastatic breast cancer, coupled with RT-PCR-based amplification of breast tumor-specific transcripts, resulted in the detection of anchorage-independent tumor cells in the majority of patients with breast cancer with known metastatic disease. CONCLUSION: Complementation of mammaglobin with three additional genes in RT-PCR increases the detection of breast cancers in tissue and circulating tumor cells.
BACKGROUND: We used a combination of genetic subtraction, silicon DNA microarray analysis, and quantitative PCR to identify tissue- and tumor-specific genes as diagnostic targets for breast cancer. METHODS AND RESULTS: From a large number of candidate antigens, several specific subsets of genes were identified that showed concordant and complementary expression profiles. Whereas transcriptional profiling of mammaglobin resulted in the detection of 70% of tumors in a panel of 46 primary and metastatic breast cancers, the inclusion of three additional markers resulted in detection of all 46 specimens. Immunomagnetic epithelial cell enrichment of circulating tumor cells from the peripheral blood of patients with metastatic breast cancer, coupled with RT-PCR-based amplification of breast tumor-specific transcripts, resulted in the detection of anchorage-independent tumor cells in the majority of patients with breast cancer with known metastatic disease. CONCLUSION: Complementation of mammaglobin with three additional genes in RT-PCR increases the detection of breast cancers in tissue and circulating tumor cells.
Authors: E Leygue; L Snell; H Dotzlaw; K Hole; S Troup; T Hiller-Hitchcock; L C Murphy; P H Watson Journal: J Pathol Date: 1999-09 Impact factor: 7.996
Authors: L Diatchenko; Y F Lau; A P Campbell; A Chenchik; F Moqadam; B Huang; S Lukyanov; K Lukyanov; N Gurskaya; E D Sverdlov; P D Siebert Journal: Proc Natl Acad Sci U S A Date: 1996-06-11 Impact factor: 11.205
Authors: V M Martin; C Siewert; A Scharl; T Harms; R Heinze; S Ohl; A Radbruch; S Miltenyi; J Schmitz Journal: Exp Hematol Date: 1998-03 Impact factor: 3.084
Authors: Barbara K Zehentner; Heather Secrist; Dawn C Hayes; Xinqun Zhang; Richard C Ostenson; Steven Loop; Gary Goodman; Raymond L Houghton; David H Persing Journal: Mol Diagn Ther Date: 2006 Impact factor: 4.074
Authors: John Backus; Todd Laughlin; Yixin Wang; Robert Belly; Robert White; Jon Baden; C Justus Min; Ann Mannie; Lorraine Tafra; David Atkins; Kathryn M Verbanac Journal: J Mol Diagn Date: 2005-08 Impact factor: 5.568
Authors: Barbara K Zehentner; Davin C Dillon; Yuqiu Jiang; Jiangchun Xu; Angela Bennington; David A Molesh; XinQun Zhang; Steven G Reed; David Persing; Raymond L Houghton Journal: Clin Chem Date: 2002-08 Impact factor: 8.327
Authors: Barbara K Zehentner; David H Persing; Amadou Deme; Papa Toure; Stephen E Hawes; Lisa Brooks; Qinghua Feng; Dawn C Hayes; Cathy W Critichlow; Raymond L Houghton; Nancy B Kiviat Journal: Clin Chem Date: 2004-09-16 Impact factor: 8.327
Authors: Manuel Valladares-Ayerbes; Pilar Iglesias-Díaz; Silvia Díaz-Prado; Daniel Ayude; Vanessa Medina; Mar Haz; Margarita Reboredo; Silvia Antolín; Lourdes Calvo; Luis M Antón-Aparicio Journal: J Cancer Res Clin Oncol Date: 2009-02-17 Impact factor: 4.553
Authors: M Mitas; K Mikhitarian; L Hoover; M A Lockett; L Kelley; A Hill; W E Gillanders; D J Cole Journal: Br J Cancer Date: 2002-03-18 Impact factor: 7.640