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Literature DB >> 11457547

Detection of anthrax vaccine virulence factors by polymerase chain reaction.

A Fasanella¹, S Losito, T Trotta, R Adone, S Massa, F Ciuchini, D Chiocco.

Abstract

In Italy, an attenuated Bacillus anthracis strain, named 'Carbosap', is used for immunization against ovine and bovine anthrax. Analysis on 'Carbosap', Sterne vaccine strain F34 and Pasteur vaccine strain SS104, were performed using primers specific for the sequences, encoding the toxic factors, located on plasmids pXO1 and pXO2 and primers specific for the chromosome. The results obtained from polymerase chain reaction (PCR) assay revealed the presence of both plasmids pXO1 and pXO2 in 'Carbosap' strain. This study showed that the 'Carbosap' vaccine strain has a different plasmid pattern in comparison to Pasteur vaccine strain SS104 and Sterne vaccine strain F34.

Entities: Species

Mesh：

Substances：
Anthrax Vaccines

Year: 2001 PMID： 11457547 DOI： 10.1016/s0264-410x(01)00159-1

Source DB: PubMed Journal: Vaccine ISSN： 0264-410X Impact factor: 3.641

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Cited

22 in total

1. Multiplex PCR for species-level identification of Bacillus anthracis and detection of pXO1, pXO2, and related plasmids.

Authors: Marco A Riojas; Katalin Kiss; Marian L McKee; Manzour Hernando Hazbón
Journal: Health Secur Date: 2015-03-20

2. Protocol for real-time PCR identification of anthrax spores from nasal swabs after broth enrichment.

Authors: Marco R Oggioni; Francesca Meacci; Alessandra Carattoli; Alessandra Ciervo; Germano Orru; Antonio Cassone; Gianni Pozzi
Journal: J Clin Microbiol Date: 2002-11 Impact factor: 5.948

3. Validation of a pXO2-A PCR assay to explore diversity among Italian isolates of Bacillus anthracis strains closely related to the live, attenuated Carbosap vaccine.

Authors: M Muscillo; G La Rosa; M Sali; E De Carolis; R Adone; F Ciuchini; A Fasanella
Journal: J Clin Microbiol Date: 2005-09 Impact factor: 5.948

Detection of anthrax vaccine virulence factors by polymerase chain reaction.

1. Multiplex PCR for species-level identification of Bacillus anthracis and detection of pXO1, pXO2, and related plasmids.

2. Protocol for real-time PCR identification of anthrax spores from nasal swabs after broth enrichment.

3. Validation of a pXO2-A PCR assay to explore diversity among Italian isolates of Bacillus anthracis strains closely related to the live, attenuated Carbosap vaccine.

4. The phenotypic and genotypic characterization of Bacillus anthracis isolates from Iran.

5. PCR assay to detect Bacillus anthracis spores in heat-treated specimens.

6. Molecular characterization of the circulating Bacillus anthracis in Jordan.

7. Evaluation of the house fly Musca domestica as a mechanical vector for an anthrax.

8. Detection of the Bacillus anthracis gyrA gene by using a minor groove binder probe.

9. Efficacy of a vaccine based on protective antigen and killed spores against experimental inhalational anthrax.

10. Anthrax in red deer (Cervus elaphus), Italy.