Literature DB >> 11447199

Localization of surface immunogenic protein on group B streptococcus.

S Rioux1, D Martin, H W Ackermann, J Dumont, J Hamel, B R Brodeur.   

Abstract

The localization and accessibility of the group B streptococcus (GBS) surface immunogenic protein (Sip) at the surface of intact GBS cells were studied by flow cytometric assay and immunogold electron microscopy. Antibodies present in pooled sera collected from mice after immunization with purified recombinant Sip efficiently recognized native Sip at the surfaces of the different GBS strains tested, which included representatives of all nine serotypes. Examination of GBS cells by immunogold electron microscopy revealed that the Sip-specific antibodies attached preferentially to polar sites and the septal region. This result confirmed that Sip is exposed at the intact-cell surface, but it also suggests that its distribution is restricted to certain regions of the cell.

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Year:  2001        PMID： 11447199      PMCID： PMC98613          DOI： 10.1128/IAI.69.8.5162-5165.2001

Source DB:  PubMed          Journal:  Infect Immun        ISSN： 0019-9567            Impact factor:   3.441


  15 in total

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Journal:  Infect Immun       Date:  2000-10       Impact factor: 3.441

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6.  Protection from group B streptococcal infection in neonatal mice by maternal immunization with recombinant Sip protein.

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