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Literature DB >> 11444602

Complete sequencing of mono-deprotonated peptide nucleic acids by sustained off-resonance irradiation collision-induced dissociation.

Abstract

Complete peptide nucleic acids (PNAs) sequence information is obtained from the unimolecular decomposition of singly-charged PNA oligomers in the negative-ion mode using electrospray ionization coupled with Fourier transform ion cyclotron resonance mass spectrometry (ESI-FTICR-MS) and sustained off-resonance irradiation collision induced dissociation. The 4-mers, n-CATT-c, n-AGCT-c, n-AACT-c, and n-acetylated-AACT-c and two 6-mers, n-AAAAAA-c and n-CCCCCC-c, were investigated to explore the unimolecular decomposition of mixed-nucleobase and homopolymer PNAs representing purine and pyrimidine oligomers, respectively. PNA decomposition is explored using a product-ion appearance curve and double resonance experiments. A decomposition mechanism for sequence ion formation (PNA amide bond cleavage) is proposed.

Entities: Chemical

Mesh：

Substances：
Peptide Nucleic Acids

Year: 2001 PMID： 11444602 DOI： 10.1016/S1044-0305(01)00259-8

Source DB: PubMed Journal: J Am Soc Mass Spectrom ISSN： 1044-0305 Impact factor: 3.262

14 in total

1. Precise mass measurement of a double-stranded 500 base-pair (309 kDa) polymerase chain reaction product by negative ion electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry.

Authors:
Journal: Rapid Commun Mass Spectrom Date: 1999-06 Impact factor: 2.419

2. Genotyping short tandem repeats using flow injection and electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry.

Authors: J C Hannis; D C Muddiman
Journal: Rapid Commun Mass Spectrom Date: 2001 Impact factor: 2.419

3. A dual electrospray ionization source combined with hexapole accumulation to achieve high mass accuracy of biopolymers in Fourier transform ion cyclotron resonance mass spectrometry.

Authors: J C Hannis; D C Muddiman
Journal: J Am Soc Mass Spectrom Date: 2000-10 Impact factor: 3.109

4. Charge-state reduction with improved signal intensity of oligonucleotides in electrospray ionization mass spectrometry.

Authors: D C Muddiman; X Cheng; H R Udseth; R D Smith
Journal: J Am Soc Mass Spectrom Date: 1996-08 Impact factor: 3.109

5. Positive-ion fast-atom bombardment tandem mass spectrometry of peptide nucleic acids.

Authors: T Takao; H Fukuda; J Coull; Y Shimonishi
Journal: Rapid Commun Mass Spectrom Date: 1994-12 Impact factor: 2.419

6. Enhanced PCR amplification of VNTR locus D1S80 using peptide nucleic acid (PNA).

Authors: D B Demers; E T Curry; M Egholm; A C Sozer
Journal: Nucleic Acids Res Date: 1995-08-11 Impact factor: 16.971

7. Detection of double-stranded PCR amplicons at the attomole level electrosprayed from low nanomolar solutions using FT-ICR mass spectrometry.

Authors: J C Hannis; D C Muddiman
Journal: Fresenius J Anal Chem Date: 2001-02

Complete sequencing of mono-deprotonated peptide nucleic acids by sustained off-resonance irradiation collision-induced dissociation.

1. Precise mass measurement of a double-stranded 500 base-pair (309 kDa) polymerase chain reaction product by negative ion electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry.

2. Genotyping short tandem repeats using flow injection and electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry.

3. A dual electrospray ionization source combined with hexapole accumulation to achieve high mass accuracy of biopolymers in Fourier transform ion cyclotron resonance mass spectrometry.

4. Charge-state reduction with improved signal intensity of oligonucleotides in electrospray ionization mass spectrometry.

5. Positive-ion fast-atom bombardment tandem mass spectrometry of peptide nucleic acids.

6. Enhanced PCR amplification of VNTR locus D1S80 using peptide nucleic acid (PNA).

7. Detection of double-stranded PCR amplicons at the attomole level electrosprayed from low nanomolar solutions using FT-ICR mass spectrometry.

8. An experimental and theoretical study of the gas-phase decomposition of monoprotonated peptide nucleic acids.

9. Utility of organic bases for improved electrospray mass spectrometry of oligonucleotides.

10. Sequence-selective recognition of DNA by strand displacement with a thymine-substituted polyamide.

1. Ion trap collision-induced dissociation of locked nucleic acids.

Review 2. The multifaceted roles of mass spectrometric analysis in nucleic acids drug discovery and development.