M Orsini1, V Romano-Spica. 1. Institute of Hygiene, Catholic University Medical School, Rome, Italy.
Abstract
AIMS: A simple and rapid method was described for DNA isolation directly from activated sludge or other environmental sources, including soil and sediments. METHODS AND RESULTS: The present method is based on microwave thermal shock and provides DNA suitable for further analysis. It is also effective for RNA extraction. CONCLUSION: The protocol is effective, easy, fast and does not require the use of expensive equipment or reagents. SIGNIFICANCE AND IMPACT OF THE STUDY: The described method can be applied to difficult substrates in environmental microbiology studies.
AIMS: A simple and rapid method was described for DNA isolation directly from activated sludge or other environmental sources, including soil and sediments. METHODS AND RESULTS: The present method is based on microwave thermal shock and provides DNA suitable for further analysis. It is also effective for RNA extraction. CONCLUSION: The protocol is effective, easy, fast and does not require the use of expensive equipment or reagents. SIGNIFICANCE AND IMPACT OF THE STUDY: The described method can be applied to difficult substrates in environmental microbiology studies.
Authors: Pascal Weigold; Mohamed El-Hadidi; Alexander Ruecker; Daniel H Huson; Thomas Scholten; Maik Jochmann; Andreas Kappler; Sebastian Behrens Journal: Sci Rep Date: 2016-06-29 Impact factor: 4.379