K Todoriki1, T Mukai, S Sato, T Toba. 1. Department of Biochemistry and Biotechnology, Faculty of Agriculture and Life Science, Hirosaki University, Bunkyocho 3, 036-8561 Hirosaki, Japan.
Abstract
AIMS: To select adhesive strains among strains of Lactobacillus and to apply them to inhibit adhesion of food-borne pathogens. METHODS AND RESULTS: Twelve Lactobacillus strains (10 from intestine) were examined for adhesion using Caco-2 cell cultures. The two most adhesive strains, Lactobacillus crispatus JCM 8779 and Lact. reuteri JCM 1081, were used to test antiadhesion activity against enterotoxigenic Escherichia coli, Salmonella typhimurium and Enterococcus faecalis strains. Adhesion of the pathogens was inhibited by both Lactobacillus strains. Adhesion of Ent. faecalis was especially strongly inhibited by JCM 8779. Although antimicrobial activity was not detected in the culture supernatant fluid by agar well diffusion assay, the supernatant fluid obtained from the harvested JCM 8779 cell suspension showed bactericidal activity against Ent. faecalis. CONCLUSION: The strong antiadhesion activity of JCM 8779 against Ent. faecalis appears to be due to the combined effect of both bactericidal activity and competition for attachment site. SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first report that Lact. crispatus produces a bactericidal substance.
AIMS: To select adhesive strains among strains of Lactobacillus and to apply them to inhibit adhesion of food-borne pathogens. METHODS AND RESULTS: Twelve Lactobacillus strains (10 from intestine) were examined for adhesion using Caco-2 cell cultures. The two most adhesive strains, Lactobacillus crispatus JCM 8779 and Lact. reuteri JCM 1081, were used to test antiadhesion activity against enterotoxigenic Escherichia coli, Salmonella typhimurium and Enterococcus faecalis strains. Adhesion of the pathogens was inhibited by both Lactobacillus strains. Adhesion of Ent. faecalis was especially strongly inhibited by JCM 8779. Although antimicrobial activity was not detected in the culture supernatant fluid by agar well diffusion assay, the supernatant fluid obtained from the harvested JCM 8779 cell suspension showed bactericidal activity against Ent. faecalis. CONCLUSION: The strong antiadhesion activity of JCM 8779 against Ent. faecalis appears to be due to the combined effect of both bactericidal activity and competition for attachment site. SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first report that Lact. crispatus produces a bactericidal substance.
Authors: Ralf Jäger; Alex E Mohr; Katie C Carpenter; Chad M Kerksick; Martin Purpura; Adel Moussa; Jeremy R Townsend; Manfred Lamprecht; Nicholas P West; Katherine Black; Michael Gleeson; David B Pyne; Shawn D Wells; Shawn M Arent; Abbie E Smith-Ryan; Richard B Kreider; Bill I Campbell; Laurent Bannock; Jonathan Scheiman; Craig J Wissent; Marco Pane; Douglas S Kalman; Jamie N Pugh; Jessica A Ter Haar; Jose Antonio Journal: J Int Soc Sports Nutr Date: 2019-12-21 Impact factor: 5.150
Authors: Rosemarie De Weirdt; Aurélie Crabbé; Stefan Roos; Sabine Vollenweider; Christophe Lacroix; Jan Peter van Pijkeren; Robert A Britton; Shameema Sarker; Tom Van de Wiele; Cheryl A Nickerson Journal: PLoS One Date: 2012-05-31 Impact factor: 3.240