Literature DB >> 1144067

Peptidyl - tRNA hydrolase and RNase activities in cell fractions of rat liver used in in vitro reconstitution of rough membrane.

A A Hochberg, H H Czosnek, E Ziv.   

Abstract

Peptidyl-tRNA hydrolase and RNase activities have been studied in those fractions of rat liver, which are used in in vitro reconstitution of rough membrane, because these enzymes may interfere with the in vitro reconstitution. It was found that smooth membrane has an active peptidyl-tRNA hydrolase, while the other fractions tested, polyribosomes, rough membrane, stripped rough membrane and the post-microsomal supernatant had no, or very low, peptidyl-tRNA hydrolase activity. Polyribosomes, rough and stripped rough membrane have RNase activity; this activity could be completely inhibited by rat liver RNase inhibitor. It is shown that RNase inhibitor is an obligatory component in in vitro experiments, in which rough membrane is reconstituted from stripped rough membrane, ribosomes and mRNA.

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Year:  1975        PMID： 1144067      PMCID： PMC343480          DOI： 10.1093/nar/2.6.943

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  20 in total

1.  Enzymatic hydrolysis of N-substituted aminoacyl-tRNA.

Authors:  D Paulin; P Yot; F Chapeville
Journal:  FEBS Lett       Date:  1968-08       Impact factor: 4.124

Review 2.  Introductory remarks: membrane-polysome interactions.

Authors:  H C Pitot; T K Shires
Journal:  Fed Proc       Date:  1973-01

Review 3.  The chemical synthesis and the biochemical properties of peptidyl-tRNA.

Authors:  Y Lapidot; N de Groot
Journal:  Prog Nucleic Acid Res Mol Biol       Date:  1972

4.  The synthesis of oligopeptidyl-tRNA.

Authors:  Y Lapidot; S Rappoport
Journal:  Methods Enzymol       Date:  1974       Impact factor: 1.600

5.  Enzymatic hydrolysis of N-substituted aminoacyl-tRNA.

Authors:  F Cuzin; N Kretchmer; R E Greenberg; R Hurwitz; F Chapeville
Journal:  Proc Natl Acad Sci U S A       Date:  1967-11       Impact factor: 11.205

6.  Assembly of membrane-bound polyribosomes.

Authors:  C Baglioni; I Bleiberg; M Zauderer
Journal:  Nat New Biol       Date:  1971-07-07

7.  Purification of rat liver RNase inhibitor and its effect on polyribosome integrity.

Authors:  A A Gribnau; J G Schoenmakers; H Bloemendal
Journal:  Arch Biochem Biophys       Date:  1969-03       Impact factor: 4.013

8.  Preferential synthesis of ferritin and albumin by different populations of liver polysomes.

Authors:  S J Hicks; J W Drysdale; H N Munro
Journal:  Science       Date:  1969-05-02       Impact factor: 47.728

9.  Biosynthesis of serum proteins and ferritin by free and attached ribosomes of rat liver.

Authors:  C M Redman
Journal:  J Biol Chem       Date:  1969-08-25       Impact factor: 5.157

10.  The wheat embryo cell-free system.

Authors:  A Marcus; D Efron; D P Weeks
Journal:  Methods Enzymol       Date:  1974       Impact factor: 1.600
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  3 in total

1.  Studies on the amino acid-incorporating activity of native rat liver rough membrane and that reconstituted in vitro.

Authors:  N De Groot; I J Yuli; H Czosnek; Y Shiklosh; A A Hochberg
Journal:  Biochem J       Date:  1976-07-15       Impact factor: 3.857

2.  Location of RNase and RNase inhibitor on free cytoplasmic mRNA-protein particles from human placenta.

Authors:  O Gileadi; H Lorberboum; N de Groot; A A Hochberg
Journal:  Mol Biol Rep       Date:  1984-01       Impact factor: 2.316

3.  RNA synthesis in cultured human placenta.

Authors:  H Lorberboum; D Weinstein; H Galski; N de Groot; S Segal; J Ilan; R Folman; A A Hochberg
Journal:  Mol Biol Rep       Date:  1982-03-31       Impact factor: 2.316
  3 in total

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