Literature DB >> 11437410

Quantitative measurement of thyroglobulin mRNA in peripheral blood of patients after total thyroidectomy.

T Takano1, A Miyauchi, H Yoshida, Y Hasegawa, K Kuma, N Amino.   

Abstract

Previous studies have reported the clinical usefulness of reverse transcription-polymerase chain reaction (RT-PCR) detection of thyroglobulin (TG) mRNA in the peripheral blood of patients with differentiated thyroid carcinoma. To evaluate this usefulness, we measured TG mRNA in the peripheral blood of patients diagnosed with thyroid carcinoma after total thyroidectomy by real-time quantitative RT-PCR using glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA as an internal control. Surprisingly, we detected TG mRNA in all samples obtained after total thyroidectomy, including those from 4 medullary carcinomas. Further, there was no statistical difference in expression levels of TG mRNA in the patients with or without metastasis, and no significant correlation was found between serum TG concentrations and the expression levels of TG mRNA. These results give rise to a question regarding the clinical applications of not only RT-PCR detection but also quantitative measurement of TG mRNA in peripheral blood. Copyright 2001 Cancer Research Campaign.

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Year:  2001        PMID： 11437410      PMCID： PMC2363919          DOI： 10.1054/bjoc.2001.1904

Source DB:  PubMed          Journal:  Br J Cancer        ISSN： 0007-0920            Impact factor:   7.640


  15 in total

1.  Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.

Authors:  P Chomczynski; N Sacchi
Journal:  Anal Biochem       Date:  1987-04       Impact factor: 3.365

2.  Alpha-fetoprotein gene expression in human lymphoblastoid cells and in PHA-stimulated normal T-lymphocytes.

Authors:  C Lafarge-Frayssinet; J M Torres; M Frain; J Uriel
Journal:  Biochem Biophys Res Commun       Date:  1989-02-28       Impact factor: 3.575

3.  Quantitative reverse transcription-PCR measurement of thyroglobulin mRNA in peripheral blood of healthy subjects.

Authors:  S T Wingo; M D Ringel; J S Anderson; A D Patel; Y D Lukes; Y Y Djuh; B Solomon; D Nicholson; P L Balducci-Silano; M A Levine; G L Francis; R M Tuttle
Journal:  Clin Chem       Date:  1999-06       Impact factor: 8.327

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Authors:  P A Singer; D S Cooper; G H Daniels; P W Ladenson; F S Greenspan; E G Levy; L E Braverman; O H Clark; I R McDougall; K V Ain; S G Dorfman
Journal:  Arch Intern Med       Date:  1996-10-28

5.  Preoperative diagnosis of thyroid papillary and anaplastic carcinomas by real-time quantitative reverse transcription-polymerase chain reaction of oncofetal fibronectin messenger RNA.

Authors:  T Takano; A Miyauchi; T Yokozawa; F Matsuzuka; I Maeda; K Kuma; N Amino
Journal:  Cancer Res       Date:  1999-09-15       Impact factor: 12.701

6.  Quantitative reverse transcription-polymerase chain reaction of circulating thyroglobulin messenger ribonucleic acid for monitoring patients with thyroid carcinoma.

Authors:  M D Ringel; P L Balducci-Silano; J S Anderson; C A Spencer; J Silverman; Y H Sparling; G L Francis; K D Burman; L Wartofsky; P W Ladenson; M A Levine; R M Tuttle
Journal:  J Clin Endocrinol Metab       Date:  1999-11       Impact factor: 5.958

7.  Detection of thyroglobulin, thyroid peroxidase, and RET/PTC1 mRNA transcripts in the peripheral blood of patients with thyroid disease.

Authors:  G Tallini; R A Ghossein; J Emanuel; J Gill; B Kinder; A B Dimich; J Costa; R Robbins; G N Burrow; J Rosai
Journal:  J Clin Oncol       Date:  1998-03       Impact factor: 44.544

8.  Molecular diagnosis of residual and recurrent thyroid cancer by amplification of thyroglobulin messenger ribonucleic acid in peripheral blood.

Authors:  M D Ringel; P W Ladenson; M A Levine
Journal:  J Clin Endocrinol Metab       Date:  1998-12       Impact factor: 5.958

9.  Detection of circulating tumor cells in patients with localized and metastatic prostatic carcinoma: clinical implications.

Authors:  R A Ghossein; H I Scher; W L Gerald; W K Kelly; T Curley; A Amsterdam; Z F Zhang; J Rosai
Journal:  J Clin Oncol       Date:  1995-05       Impact factor: 44.544

10.  Molecular detection of thyroglobulin mRNA transcripts in peripheral blood of patients with thyroid disease by RT-PCR.

Authors:  J Bojunga; S Röddiger; M Stanisch; K Kusterer; R Kurek; H Renneberg; S Adams; E Lindhorst; K H Usadel; P M Schumm-Draeger
Journal:  Br J Cancer       Date:  2000-05       Impact factor: 7.640
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  5 in total

1.  Preoperative determination of serum thyroglobulin to identify patients with differentiated thyroid cancer who may present recurrence without increased thyroglobulin.

Authors:  B Gibelli; P Tredici; C De Cicco; L Bodei; M T Sandri; G Renne; R Bruschini; N Tradati
Journal:  Acta Otorhinolaryngol Ital       Date:  2005-04       Impact factor: 2.124

2.  Human kallikrein 2 (hK2) mRNA in peripheral blood of patients with thyroid cancer: a novel molecular marker?

Authors:  Sandra J Roddiger; Heiner Renneberg; Thomas Martin; Ulf W Tunn; Nikolaos Zamboglou; Ralf Kurek
Journal:  J Cancer Res Clin Oncol       Date:  2003-02-12       Impact factor: 4.553

3.  Peripheral blood levels of thyroglobulin mRNA and serum thyroglobulin concentrations after radioiodine ablation of multinodular goiter with or without pre-treatment with recombinant human thyrotropin.

Authors:  I G S Rubio; M N C Silva; M Knobel; R Romão; R Possato; E M M S Gebrin; C Buchpiguel; G Medeiros-Neto
Journal:  J Endocrinol Invest       Date:  2007 Jul-Aug       Impact factor: 4.256

4.  Oncofoetal fibronectin--a tumour-specific marker in detecting minimal residual disease in differentiated thyroid carcinoma.

Authors:  E Hesse; P B Musholt; E Potter; T Petrich; M Wehmeier; R von Wasielewski; R Lichtinghagen; T J Musholt
Journal:  Br J Cancer       Date:  2005-09-05       Impact factor: 7.640

Review 5.  Detection of circulating Tg-mRNA in the follow-up of papillary and follicular thyroid cancer: how useful is it?

Authors:  F A Verburg; C J M Lips; E G W M Lentjes; J M H de Klerk
Journal:  Br J Cancer       Date:  2004-07-19       Impact factor: 7.640
  5 in total

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