Literature DB >> 11425755

Development of a quantitative competitive PCR assay for detection and quantification of Escherichia coli O157:H7 cells.

W Li1, M A Drake.   

Abstract

A quantitative competitive PCR (QC-PCR) assay was developed to detect and quantify Escherichia coli O157:H7 cells. From 10(3) to 10(8) CFU of E. coli O157:H7 cells/ml was quantified in broth or skim milk, and cell densities predicted by QC-PCR were highly related to viable cell counts (r(2) = 0.99 and 0.93, respectively). QC-PCR has potential for quantitative detection of pathogenic bacteria in foods.

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Year:  2001        PMID： 11425755      PMCID： PMC93014          DOI： 10.1128/AEM.67.7.3291-3294.2001

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  17 in total

Review 1.  Escherichia coli O157:H7 as an emerging foodborne pathogen: a literature review.

Authors:  S Park; R W Worobo; R A Durst
Journal:  Crit Rev Food Sci Nutr       Date:  1999-11       Impact factor: 11.176

2.  Competitive PCR.

Authors:  P D Siebert; J W Larrick
Journal:  Nature       Date:  1992-10-08       Impact factor: 49.962

3.  Nucleic acid persistence in heat-killed Escherichia coli O157:H7 from contaminated skim milk.

Authors:  J L McKillip; L A Jaykus; M Drake
Journal:  J Food Prot       Date:  1999-08       Impact factor: 2.077

4.  Quantitative competitive polymerase chain reaction for accurate quantitation of HIV DNA and RNA species.

Authors:  M Piatak; K C Luk; B Williams; J D Lifson
Journal:  Biotechniques       Date:  1993-01       Impact factor: 1.993

5.  Rapid construction of deleted DNA fragments for use as internal standards in competitive PCR.

Authors:  C F Jin; M Mata; D J Fink
Journal:  PCR Methods Appl       Date:  1994-02

6.  Quantitative PCR: theoretical considerations with practical implications.

Authors:  L Raeymaekers
Journal:  Anal Biochem       Date:  1993-11-01       Impact factor: 3.365

7.  Cloning and sequencing of a Shiga-like toxin II-related gene from Escherichia coli O157:H7 strain 7279.

Authors:  T Meyer; H Karch; J Hacker; H Bocklage; J Heesemann
Journal:  Zentralbl Bakteriol       Date:  1992-01

8.  Properties of strains of Escherichia coli belonging to serogroup O157 with special reference to production of Vero cytotoxins VT1 and VT2.

Authors:  S M Scotland; G A Willshaw; H R Smith; B Rowe
Journal:  Epidemiol Infect       Date:  1987-12       Impact factor: 2.451

9.  Acute renal tubular necrosis and death of mice orally infected with Escherichia coli strains that produce Shiga-like toxin type II.

Authors:  E A Wadolkowski; L M Sung; J A Burris; J E Samuel; A D O'Brien
Journal:  Infect Immun       Date:  1990-12       Impact factor: 3.441

10.  Mathematical considerations of competitive polymerase chain reaction.

Authors:  A R Connolly; L G Cleland; B W Kirkham
Journal:  J Immunol Methods       Date:  1995-12-01       Impact factor: 2.303
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  2 in total

1.  Effect of stress on the ability of a phlA-based quantitative competitive PCR assay to monitor biocontrol strain Pseudomonas fluorescens CHA0.

Authors:  Fabio Rezzonico; Yvan Moënne-Loccoz; Geneviève Défago
Journal:  Appl Environ Microbiol       Date:  2003-01       Impact factor: 4.792

2.  Growth of a Dehalococcoides-like microorganism on vinyl chloride and cis-dichloroethene as electron acceptors as determined by competitive PCR.

Authors:  Alison M Cupples; Alfred M Spormann; Perry L McCarty
Journal:  Appl Environ Microbiol       Date:  2003-02       Impact factor: 4.792
  2 in total

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