Literature DB >> 11420448

Application of high-precision isotope ratio monitoring mass spectrometry to identify the biosynthetic origins of proteins.

I Apostol1, P D Brooks, A J Mathews.   

Abstract

Isotope ratio monitoring (IRM) mass spectrometry was used to measure the relative abundance of stable isotopes in several samples of adult human hemoglobin expressed in E. coli, yeast, and human blood. The results showed significant differences in the distribution of (15)N and (13)C isotopes among hemoglobin samples produced in these organisms. This indicates that IRM mass spectrometry can be used in forensic protein chemistry to identify the origin of protein expression.

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Year:  2001        PMID： 11420448      PMCID： PMC2374111          DOI： 10.1110/ps.90101

Source DB:  PubMed          Journal:  Protein Sci        ISSN： 0961-8368            Impact factor:   6.725


  27 in total

1.  High precision isotope ratio monitoring techniques in mass spectrometry.

Authors:  W A Brand
Journal:  J Mass Spectrom       Date:  1996-03       Impact factor: 1.982

2.  Structural characterization of human hemoglobin crosslinked by bis(3,5-dibromosalicyl) fumarate using mass spectrometric techniques.

Authors:  Z Yu; G Friso; J J Miranda; M J Patel; T Lo-Tseng; E G Moore; A L Burlingame
Journal:  Protein Sci       Date:  1997-12       Impact factor: 6.725

3.  Human haemoglobin from transgenic tobacco.

Authors:  W Dieryck; J Pagnier; C Poyart; M C Marden; V Gruber; P Bournat; S Baudino; B Merot
Journal:  Nature       Date:  1997-03-06       Impact factor: 49.962

4.  Production of human normal adult and fetal hemoglobins in Escherichia coli.

Authors:  T J Shen; N T Ho; M Zou; D P Sun; P F Cottam; V Simplaceanu; M F Tam; D A Bell; C Ho
Journal:  Protein Eng       Date:  1997-09

5.  Isotope ratio mass spectrometry, compared with conventional mass spectrometry in kinetic studies at low and high enrichment levels: application to lipoprotein kinetics.

Authors:  F Pont; L Duvillard; C Maugeais; A Athias; L Perségol; P Gambert; B Vergès
Journal:  Anal Biochem       Date:  1997-06-01       Impact factor: 3.365

6.  Increased production of peptide deformylase eliminates retention of formylmethionine in bovine somatotropin overproduced in Escherichia coli.

Authors:  W C Warren; K A Bentle; M R Schlittler; A C Schwane; J P O'Neil; G Bogosian
Journal:  Gene       Date:  1996-10-03       Impact factor: 3.688

7.  Expression of recombinant human hemoglobin in Escherichia coli.

Authors:  D Looker; A J Mathews; J O Neway; G L Stetler
Journal:  Methods Enzymol       Date:  1994       Impact factor: 1.600

8.  Incorporation of norvaline at leucine positions in recombinant human hemoglobin expressed in Escherichia coli.

Authors:  I Apostol; J Levine; J Lippincott; J Leach; E Hess; C B Glascock; M J Weickert; R Blackmore
Journal:  J Biol Chem       Date:  1997-11-14       Impact factor: 5.157

9.  Properties of a recombinant human hemoglobin with aspartic acid 99(beta), an important intersubunit contact site, substituted by lysine.

Authors:  H Yanase; S Cahill; J J Martin de Llano; L R Manning; K Schneider; B T Chait; K D Vandegriff; R M Winslow; J M Manning
Journal:  Protein Sci       Date:  1994-08       Impact factor: 6.725

10.  Improved method of detection of testosterone abuse by gas chromatography/combustion/isotope ratio mass spectrometry analysis of urinary steroids.

Authors:  R Aguilera; M Becchi; H Casabianca; C K Hatton; D H Catlin; B Starcevic; H G Pope
Journal:  J Mass Spectrom       Date:  1996-02       Impact factor: 1.982
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  1 in total

1.  Statistical Approaches to Assess Biosimilarity from Analytical Data.

Authors:  Richard Burdick; Todd Coffey; Hiten Gutka; Gyöngyi Gratzl; Hugh D Conlon; Chi-Ting Huang; Michael Boyne; Henriette Kuehne
Journal:  AAPS J       Date:  2016-10-05       Impact factor: 4.009
  1 in total

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