| Literature DB >> 11418670 |
A A Kaspar1, S Okada, J Kumar, F R Poulain, K A Drouvalakis, A Kelekar, D A Hanson, R M Kluck, Y Hitoshi, D E Johnson, C J Froelich, C B Thompson, D D Newmeyer, A Anel, C Clayberger, A M Krensky.
Abstract
Granulysin is an antimicrobial and tumoricidal molecule expressed in granules of CTL and NK cells. In this study, we show that granulysin damages cell membranes based upon negative charge, disrupts the transmembrane potential (Deltapsi) in mitochondria, and causes release of cytochrome c. Granulysin-induced apoptosis is blocked in cells overexpressing Bcl-2. Despite the release of cytochrome c, procaspase 9 is not processed. Nevertheless, activation of caspase 3 is observed in granulysin-treated cells, suggesting that granulysin activates a novel pathway of CTL- and NK cell-mediated death distinct from granzyme- and death receptor-induced apoptosis.Entities:
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Year: 2001 PMID： 11418670 DOI： 10.4049/jimmunol.167.1.350
Source DB: PubMed Journal: J Immunol ISSN： 0022-1767 Impact factor: 5.422