Literature DB >> 11418499

Detection and removal of an artefact fatty acid from the binding site of recombinant Bombyx mori pheromone-binding protein.

N J Oldham1, J Krieger, H Breer, A Svatos.   

Abstract

Recombinant Bombyx mori pheromone-binding protein (PBP), purified from an Escherichia coli expression system, has been found to contain (11Z)-octadecenoic acid (cis-vaccenic acid) as an artefact ligand. An efficient delipidation procedure is described to overcome what would appear to be a general problem with recombinant lepidopteran PBPs.

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Year:  2001        PMID： 11418499     DOI： 10.1093/chemse/26.5.529

Source DB:  PubMed          Journal:  Chem Senses        ISSN： 0379-864X            Impact factor:   3.160


  8 in total

1.  Major venom proteins of the fire ant Solenopsis invicta: insights into possible pheromone-binding function from mass spectrometric analysis.

Authors:  T Das; I Alabi; M Colley; F Yan; W Griffith; S Bach; S T Weintraub; R Renthal
Journal:  Insect Mol Biol       Date:  2018-04-15       Impact factor: 3.585

2.  Ligand binding turns moth pheromone-binding protein into a pH sensor: effect on the Antheraea polyphemus PBP1 conformation.

Authors:  Uma V Katre; Suman Mazumder; Rabi K Prusti; Smita Mohanty
Journal:  J Biol Chem       Date:  2009-09-16       Impact factor: 5.157

3.  Structure of Vibrio cholerae ToxT reveals a mechanism for fatty acid regulation of virulence genes.

Authors:  Michael J Lowden; Karen Skorupski; Maria Pellegrini; Michael G Chiorazzo; Ronald K Taylor; F Jon Kull
Journal:  Proc Natl Acad Sci U S A       Date:  2010-02-01       Impact factor: 11.205

4.  Ligand binding to six recombinant pheromone-binding proteins of Antheraea polyphemus and Antheraea pernyi.

Authors:  R Maida; G Ziegelberger; K-E Kaissling
Journal:  J Comp Physiol B       Date:  2003-07-23       Impact factor: 2.200

5.  Bombyx mori pheromone-binding protein binding nonpheromone ligands: implications for pheromone recognition.

Authors:  Catherine Lautenschlager; Walter S Leal; Jon Clardy
Journal:  Structure       Date:  2007-09       Impact factor: 5.006

6.  Quantitative analysis of pheromone-binding protein specificity.

Authors:  S Katti; N Lokhande; D González; A Cassill; R Renthal
Journal:  Insect Mol Biol       Date:  2012-11-01       Impact factor: 3.585

7.  A receptor and binding protein interplay in the detection of a distinct pheromone component in the silkmoth Antheraea polyphemus.

Authors:  Maike Forstner; Heinz Breer; Jürgen Krieger
Journal:  Int J Biol Sci       Date:  2009-12-03       Impact factor: 6.580

8.  Plant odorants interfere with detection of sex pheromone signals by male Heliothis virescens.

Authors:  Pablo Pregitzer; Marco Schubert; Heinz Breer; Bill S Hansson; Silke Sachse; Jürgen Krieger
Journal:  Front Cell Neurosci       Date:  2012-10-08       Impact factor: 5.505
  8 in total

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