Effects of Opuntia megacantha leaves extract on renal electrolyte and fluid handling in streptozotocin (STZ)-diabetic rats.

We previously demonstrated that Opuntia megacantha leaves' extracts can reduce blood glucose levels in diabetes mellitus. For O. megacantha leaves' extracts to have potential in the management of diabetes mellitus, it is necessary to establish its detailed effects on renal function since diabetes is associated with renal fluid and electrolyte disturbances. Therefore, the current study was designed to investigate the influence of the extracts on renal function in male diabetic Sprague-Dawley rats. Rats were made diabetic by an i.p. injection of streptozotocin (STZ, 60 mg/kg in citrate buffer). Vehicle injected animals acted as controls. Separate groups of nondiabetic and diabetic rats were orally administered O. megacantha leaves extracts (20 mg/100 g bw) or normal saline (0.1 ml x 100 g(-1) bw) daily for 5 weeks. Urine volume and total urinary outputs of Na+ and K+ were determined from 24 h samples. O. megacantha leaves' extracts significantly (p < 0.01) increased urinary Na+ output in diabetic and nondiabetic rats resulting in significantly (p < 0.01) low plasma concentration by comparison with untreated animals. Treatment with the extract significantly increased FE(Na+) and GFR in all groups. The urinary K+ outputs in nondiabetic was slightly lowered, but did not reach statistically significance. O. megacantha leaves' extracts did not alter plasma aldosterone and AVP concentrations in diabetic and nondiabetic rats in nondiabetic animals. It is concluded that O. megacantha leaves extracts modulate renal water and sodium handling. The mechanisms are not clear.