Literature DB >> 11391806

Mass spectrometric studies of the path of carbon in photosynthesis: positional isotopic analysis of (13)C-labelled C(4) to C(7) sugar phosphates.

J K MacLeod1, I L Flanigan, J F Williams, J G Collins.   

Abstract

The (EIMS) electron ionization mass spectrometric fragmentation patterns of the methoxime- and ethoxime-trimethylsilyl (TMS) derivatives of C(4) to C(7) sugars involved as phosphates in the Calvin pathway of photosynthesis in plants were analysed by gas chromatography/EIMS using specifically labelled (13)C analogs. In general, most but not all of the major ions in the mass spectra arise from single carbon-carbon bond cleavages of the straight-chain derivatives. The results confirm that GC/MS of the alkoxime-TMS derivatives is a viable method for measuring (13)C incorporations at individual carbon atoms in each of the sugar phosphates during photosynthetic experiments with (13)CO(2). Copyright 2001 John Wiley & Sons, Ltd.

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Year:  2001        PMID： 11391806     DOI： 10.1002/jms.147

Source DB:  PubMed          Journal:  J Mass Spectrom        ISSN： 1076-5174            Impact factor:   1.982


  6 in total

1.  Changes in transcript abundance in Chlamydomonas reinhardtii following nitrogen deprivation predict diversion of metabolism.

Authors:  Rachel Miller; Guangxi Wu; Rahul R Deshpande; Astrid Vieler; Katrin Gärtner; Xiaobo Li; Eric R Moellering; Simone Zäuner; Adam J Cornish; Bensheng Liu; Blair Bullard; Barbara B Sears; Min-Hao Kuo; Eric L Hegg; Yair Shachar-Hill; Shin-Han Shiu; Christoph Benning
Journal:  Plant Physiol       Date:  2010-10-08       Impact factor: 8.340

2.  The metabolic significance of octulose phosphates in the photosynthetic carbon reduction cycle in spinach.

Authors:  John F Williams; John K MacLeod
Journal:  Photosynth Res       Date:  2006-12-08       Impact factor: 3.573

3.  Subcellular localization and enzymatic properties of differentially expressed transketolase genes isolated from the desiccation tolerant resurrection plant Craterostigma plantagineum.

Authors:  Björn C Willige; Michael Kutzer; Felix Tebartz; Dorothea Bartels
Journal:  Planta       Date:  2008-12-04       Impact factor: 4.116

4.  Metabolic fluxes in an illuminated Arabidopsis rosette.

Authors:  Marek Szecowka; Robert Heise; Takayuki Tohge; Adriano Nunes-Nesi; Daniel Vosloh; Jan Huege; Regina Feil; John Lunn; Zoran Nikoloski; Mark Stitt; Alisdair R Fernie; Stéphanie Arrivault
Journal:  Plant Cell       Date:  2013-02-26       Impact factor: 11.277

5.  A re-investigation of the path of carbon in photosynthesis utilizing GC/MS methodology. Unequivocal verification of the participation of octulose phosphates in the pathway.

Authors:  Ian L Flanigan; John K MacLeod; John F Williams
Journal:  Photosynth Res       Date:  2006-12-06       Impact factor: 3.573

6.  Metabolic flux analysis of heterotrophic growth in Chlamydomonas reinhardtii.

Authors:  Nanette R Boyle; Neelanjan Sengupta; John A Morgan
Journal:  PLoS One       Date:  2017-05-24       Impact factor: 3.240
  6 in total

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