Literature DB >> 11390070

General expression of RoTat 1.2 variable antigen type in Trypanosoma evansi isolates from different origin.

D Verloo1, E Magnus, P Büscher.   

Abstract

The variable surface glycoprotein of Trypanosoma evansi RoTat 1.2 variable antigen type (VAT) is used as an antigen in different antibody detection assays for T. evansi. To obtain more information on the predominant character of RoTat 1.2 and its diagnostic potential in antibody detection tests, we checked its expression in 10 different T. evansi stocks and clones from different parts of the world. Cryostabilates were injected into mice and the trypanosomes of the first peak parasitaemia were screened for the presence of RoTat 1.2 by VAT specific immunofluorescence. To monitor the appearance of RoTat 1.2 specific antibodies during infection, rabbits were infected and serologically tested at different time intervals with VAT specific immune trypanolysis, CATT/T. evansi, LATEX/T. evansi and ELISA/T. evansi. Test results confirm the predominant character of RoTat 1.2.

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Year:  2001        PMID： 11390070     DOI： 10.1016/s0304-4017(01)00412-5

Source DB:  PubMed          Journal:  Vet Parasitol        ISSN： 0304-4017            Impact factor:   2.738


  18 in total

1.  First report of molecular characterization and phylogenetic analysis of RoTat 1.2 VSG of Trypanosoma evansi from equine isolate.

Authors:  Vikrant Sudan; Amit Kumar Jaiswal; Daya Shanker; Amit Kumar Verma
Journal:  Trop Anim Health Prod       Date:  2017-08-22       Impact factor: 1.559

2.  Evaluation of diagnostic tests for Trypanosoma evansi in experimentally infected pigs and subsequent use in field surveys in north Vietnam and Thailand.

Authors:  W G Holland; N G Thanh; T T Do; S Sangmaneedet; B Goddeeris; J Vercruysse
Journal:  Trop Anim Health Prod       Date:  2005-08       Impact factor: 1.559

3.  Detection of Trypanosoma Infection in Dromedary Camels by Using Different Diagnostic Techniques in Northern Oman.

Authors:  Amal Al-Kharusi; Elshafie Ibrahim Elshafie; Senan Baqir; Asim Faraz; Aliya Al-Ansari; Pamela Burger; Osman Mahgoub; Kaadhia Al-Kharousi; Halima Al-Duhli; Mohammed Al-Sinani; Raqiya Al-Hatali; Derek Roberts
Journal:  Animals (Basel)       Date:  2022-05-25       Impact factor: 3.231

4.  Towards a new reference test for surra in camels.

Authors:  Thao Tran; Filip Claes; Didier Verloo; Henri De Greve; Philippe Büscher
Journal:  Clin Vaccine Immunol       Date:  2009-04-29

5.  Development of a loop-mediated isothermal amplification assay based on RoTat1.2 gene for detection of Trypanosoma evansi in domesticated animals.

Authors:  Binod Kumar; Biswa Ranjan Maharana; Nilima N Brahmbhatt; Bhupendrakumar J Thakre; Vijay L Parmar
Journal:  Parasitol Res       Date:  2021-03-13       Impact factor: 2.289

6.  Molecular epidemiology of camel trypanosomiasis based on ITS1 rDNA and RoTat 1.2 VSG gene in the Sudan.

Authors:  Bashir Salim; Mohammed A Bakheit; Joseph Kamau; Ichiro Nakamura; Chihiro Sugimoto
Journal:  Parasit Vectors       Date:  2011-03-04       Impact factor: 3.876

7.  Variant surface glycoproteins from Venezuelan trypanosome isolates are recognized by sera from animals infected with either Trypanosoma evansi or Trypanosoma vivax.

Authors:  Rocío Camargo; Adriana Izquier; Graciela L Uzcanga; Trina Perrone; Alvaro Acosta-Serrano; Liomary Carrasquel; Laura P Arias; José L Escalona; Vanessa Cardozo; José Bubis
Journal:  Vet Parasitol       Date:  2014-11-13       Impact factor: 2.738

8.  Epidemiology of Trypanosoma evansi and Trypanosoma vivax in domestic animals from selected districts of Tigray and Afar regions, Northern Ethiopia.

Authors:  Hadush Birhanu; Regassa Fikru; Mussa Said; Weldu Kidane; Tadesse Gebrehiwot; Ashenafi Hagos; Tola Alemu; Tesfaye Dawit; Dirk Berkvens; Bruno Maria Goddeeris; Philippe Büscher
Journal:  Parasit Vectors       Date:  2015-04-09       Impact factor: 3.876

9.  Variable Surface Glycoprotein RoTat 1.2 PCR as a specific diagnostic tool for the detection of Trypanosoma evansi infections.

Authors:  Filip Claes; Magda Radwanska; Toyo Urakawa; Phelix Ao Majiwa; Bruno Goddeeris; Philip Büscher
Journal:  Kinetoplastid Biol Dis       Date:  2004-09-17

10.  Parasitological, serological and molecular survey of Trypanosoma evansi infection in dromedary camels from Cholistan Desert, Pakistan.

Authors:  Sonia Tehseen; Nusrat Jahan; Muhammad Fiaz Qamar; Marc Desquesnes; Mirza Imran Shahzad; Stijn Deborggraeve; Philippe Büscher
Journal:  Parasit Vectors       Date:  2015-08-12       Impact factor: 3.876
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