Literature DB >> 11389835

Structure of the replicating complex of a pol alpha family DNA polymerase.

M C Franklin1, J Wang, T A Steitz.   

Abstract

We describe the 2.6 A resolution crystal structure of RB69 DNA polymerase with primer-template DNA and dTTP, capturing the step just before primer extension. This ternary complex structure in the human DNA polymerase alpha family shows a 60 degrees rotation of the fingers domain relative to the apo-protein structure, similar to the fingers movement in pol I family polymerases. Minor groove interactions near the primer 3' terminus suggest a common fidelity mechanism for pol I and pol alpha family polymerases. The duplex product DNA orientation differs by 40 degrees between the polymerizing mode and editing mode structures. The role of the thumb in this DNA motion provides a model for editing in the pol alpha family.

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Year:  2001        PMID： 11389835     DOI： 10.1016/s0092-8674(01)00367-1

Source DB:  PubMed          Journal:  Cell        ISSN： 0092-8674            Impact factor:   41.582


  255 in total

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Authors:  Andrew F Gardner; William E Jack
Journal:  Nucleic Acids Res       Date:  2002-01-15       Impact factor: 16.971

2.  Function of the C-terminus of phi29 DNA polymerase in DNA and terminal protein binding.

Authors:  Verónica Truniger; José M Lázaro; Margarita Salas
Journal:  Nucleic Acids Res       Date:  2004-01-16       Impact factor: 16.971

3.  Differences in replication of a DNA template containing an ethyl phosphotriester by T4 DNA polymerase and Escherichia coli DNA polymerase I.

Authors:  Laura Tsujikawa; Michael Weinfield; Linda J Reha-Krantz
Journal:  Nucleic Acids Res       Date:  2003-09-01       Impact factor: 16.971

4.  Role of the LEXE motif of protein-primed DNA polymerases in the interaction with the incoming nucleotide.

Authors:  Eugenia Santos; José M Lázaro; Patricia Pérez-Arnaiz; Margarita Salas; Miguel de Vega
Journal:  J Biol Chem       Date:  2013-12-09       Impact factor: 5.157

5.  Selection and characterization of Autographa californica multiple nucleopolyhedrovirus DNA polymerase mutations.

Authors:  Guozhong Feng; David K Thumbi; Jondavid de Jong; Jeffrey J Hodgson; Basil M Arif; Daniel Doucet; Peter J Krell
Journal:  J Virol       Date:  2012-10-03       Impact factor: 5.103

6.  Mutator alleles of yeast DNA polymerase zeta.

Authors:  Ayako N Sakamoto; Jana E Stone; Grace E Kissling; Scott D McCulloch; Youri I Pavlov; Thomas A Kunkel
Journal:  DNA Repair (Amst)       Date:  2007-08-21

7.  A coordinated temporal interplay of nucleosome reorganization factor, sister chromatin cohesion factor, and DNA polymerase alpha facilitates DNA replication.

Authors:  Yanjiao Zhou; Teresa S-F Wang
Journal:  Mol Cell Biol       Date:  2004-11       Impact factor: 4.272

8.  NFI and Oct-1 bend the Ad5 origin in the same direction leading to optimal DNA replication.

Authors:  Monika E Mysiak; Claire Wyman; P Elly Holthuizen; Peter C van der Vliet
Journal:  Nucleic Acids Res       Date:  2004-12-01       Impact factor: 16.971

9.  Activity and fidelity of human DNA polymerase α depend on primer structure.

Authors:  Andrey G Baranovskiy; Vincent N Duong; Nigar D Babayeva; Yinbo Zhang; Youri I Pavlov; Karen S Anderson; Tahir H Tahirov
Journal:  J Biol Chem       Date:  2018-03-19       Impact factor: 5.157

Review 10.  Directed polymerase evolution.

Authors:  Tingjian Chen; Floyd E Romesberg
Journal:  FEBS Lett       Date:  2013-11-05       Impact factor: 4.124
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