| Literature DB >> 11368760 |
S Bolsover1, O Ibrahim, N O'luanaigh, H Williams, S Cockcroft.
Abstract
We have studied the degree to which fluorescent Ca(2+) indicator dyes, and green fluorescent protein and its variants, can be used together. We find that the most commonly used fluorescent protein, enhanced green fluorescent protein (EGFP), seriously contaminates fura 2 signals. We suggest two alternative combinations for which there is no detectable contamination of the Ca(2+) indicator signal by the fluorescent protein. Blue fluorescent protein can be used with the Ca(2+) indicator Fura Red; EGFP can be used with the Ca(2+) indicator X-Rhod 1. The use of these combinations will permit the accurate measurement of Ca(2+) signals in cells transfected with fluorescent proteins.Entities:
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Year: 2001 PMID： 11368760 PMCID： PMC1221844 DOI： 10.1042/0264-6021:3560345
Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857