A A Aroutcheva1, J A Simoes, S Faro. 1. Department of Obstetrics and Gynecology, Rush-Presbyterian-St. Luke's Medical Center, Chicago, IL 60612, USA.
Abstract
OBJECTIVE: To isolate bacteriocin from a vaginal strain of Lactobacillus acidophilus. METHODS: L. acidophilus 160 was grown on two media. The first was MRS broth for 18 hours; the cells were harvested, washed, and placed into a chemically defined medium. The second medium resembled vaginal fluid minus protein. Bacteriocin was precipitated from both media using ammonium sulfate. The growth-inhibiting activity of bacteriocin was determined by a bioassay using nine different isolates of Gardnerella vaginalis. RESULTS: MRS broth is not a suitable medium for extracting bacteriocin, because it binds with Tween 80. Bacteriocin was isolated, without contaminating constituents, from chemically defined medium and identified as a single band by electrophoresis. Bacteriocin has a molecular weight of 3.8 kDa. All nine isolates of Gardnerella were inhibited by the bacteriocin isolated from L. acidophilus 160. CONCLUSIONS: Bacteriocin produced by L. acidophilus 160 was isolated from the chemically defined medium (starvation medium) in a partially pure form. L. acidophilus 160 bacteriocin inhibited growth of all nine isolates of Gardnerella vaginalis.
OBJECTIVE: To isolate bacteriocin from a vaginal strain of Lactobacillus acidophilus. METHODS:L. acidophilus 160 was grown on two media. The first was MRS broth for 18 hours; the cells were harvested, washed, and placed into a chemically defined medium. The second medium resembled vaginal fluid minus protein. Bacteriocin was precipitated from both media using ammonium sulfate. The growth-inhibiting activity of bacteriocin was determined by a bioassay using nine different isolates of Gardnerella vaginalis. RESULTS: MRS broth is not a suitable medium for extracting bacteriocin, because it binds with Tween 80. Bacteriocin was isolated, without contaminating constituents, from chemically defined medium and identified as a single band by electrophoresis. Bacteriocin has a molecular weight of 3.8 kDa. All nine isolates of Gardnerella were inhibited by the bacteriocin isolated from L. acidophilus 160. CONCLUSIONS: Bacteriocin produced by L. acidophilus 160 was isolated from the chemically defined medium (starvation medium) in a partially pure form. L. acidophilus 160 bacteriocin inhibited growth of all nine isolates of Gardnerella vaginalis.
Authors: Yevgeniy Turovskiy; Richard D Ludescher; Alla A Aroutcheva; Sebastian Faro; Michael L Chikindas Journal: Probiotics Antimicrob Proteins Date: 2009-01-20 Impact factor: 4.609
Authors: María Silvina Juárez Tomás; Elena Bru; Birgitt Wiese; María Elena F Nader-Macías Journal: Probiotics Antimicrob Proteins Date: 2010-03 Impact factor: 4.609
Authors: Diane C Dezwaan; Michael J Mequio; Julia S Littell; Jonathan P Allen; Silvia Rossbach; Vivien Pybus Journal: Microb Ecol Health Dis Date: 2007-07-30