Literature DB >> 11350040

Development of a tRNA-dependent in vitro translation system.

R J Jackson1, S Napthine, I Brierley.   

Abstract

A method is described for depleting rabbit reticulocyte lysates and wheat germ extracts of endogenous tRNAs by affinity chromatography using a matrix generated by coupling ethanolamine to epoxy-activated Sepharose 6B. Greater than 90% depletion of tRNA is achieved with the result that translation becomes in effect absolutely dependent on added tRNA. This depletion procedure should prove very useful for studying the influence of tRNA concentration, and the spectrum of the tRNA population, on recoding events such as programmed frameshifting and readthrough of termination codons.

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Year:  2001        PMID： 11350040      PMCID： PMC1370128          DOI： 10.1017/s1355838201002539

Source DB:  PubMed          Journal:  RNA        ISSN： 1355-8382            Impact factor:   4.942


  13 in total

1.  Messenger RNA translation in the presence of homologous and heterologous tRNA.

Authors:  M A Le Meur; P Gerlinger; J P Ebel
Journal:  Eur J Biochem       Date:  1976-08-16

2.  Preparation and use of nuclease-treated rabbit reticulocyte lysates for the translation of eukaryotic messenger RNA.

Authors:  R J Jackson; T Hunt
Journal:  Methods Enzymol       Date:  1983       Impact factor: 1.600

3.  Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.

Authors:  D A Melton; P A Krieg; M R Rebagliati; T Maniatis; K Zinn; M R Green
Journal:  Nucleic Acids Res       Date:  1984-09-25       Impact factor: 16.971

4.  The use of affinity chromatography on 2'5' ADP-sepharose reveals a requirement for NADPH, thioredoxin and thioredoxin reductase for the maintenance of high protein synthesis activity in rabbit reticulocyte lysates.

Authors:  T Hunt; P Herbert; E A Campbell; C Delidakis; R J Jackson
Journal:  Eur J Biochem       Date:  1983-03-15

5.  The preparation and properties of gel-filtered rabbit-reticulocyte lysate protein-synthesis systems.

Authors:  R J Jackson; E A Campbell; P Herbert; T Hunt
Journal:  Eur J Biochem       Date:  1983-03-15

6.  The roles of sugar phosphates and thiol-reducing systems in the control of reticulocyte protein synthesis.

Authors:  R J Jackson; P Herbert; E A Campbell; T Hunt
Journal:  Eur J Biochem       Date:  1983-03-15

7.  The influence of viral coding sequences on the efficiency of internal initiation of translation of cardiovirus RNAs.

Authors:  S L Hunt; A Kaminski; R J Jackson
Journal:  Virology       Date:  1993-12       Impact factor: 3.616

8.  The Q-base of asparaginyl-tRNA is dispensable for efficient -1 ribosomal frameshifting in eukaryotes.

Authors:  B Marczinke; T Hagervall; I Brierley
Journal:  J Mol Biol       Date:  2000-01-14       Impact factor: 5.469

9.  An efficient ribosomal frame-shifting signal in the polymerase-encoding region of the coronavirus IBV.

Authors:  I Brierley; M E Boursnell; M M Binns; B Bilimoria; V C Blok; T D Brown; S C Inglis
Journal:  EMBO J       Date:  1987-12-01       Impact factor: 11.598

10.  Characterization of an efficient coronavirus ribosomal frameshifting signal: requirement for an RNA pseudoknot.

Authors:  I Brierley; P Digard; S C Inglis
Journal:  Cell       Date:  1989-05-19       Impact factor: 41.582
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  8 in total

1.  In vitro selection for sense codon suppression.

Authors:  Adam Frankel; Richard W Roberts
Journal:  RNA       Date:  2003-07       Impact factor: 4.942

2.  Mammalian ALKBH8 possesses tRNA methyltransferase activity required for the biogenesis of multiple wobble uridine modifications implicated in translational decoding.

Authors:  Lene Songe-Møller; Erwin van den Born; Vibeke Leihne; Cathrine B Vågbø; Terese Kristoffersen; Hans E Krokan; Finn Kirpekar; Pål Ø Falnes; Arne Klungland
Journal:  Mol Cell Biol       Date:  2010-02-01       Impact factor: 4.272

Review 3.  Codon-Reduced Protein Synthesis With Manipulating tRNA Components in Cell-Free System.

Authors:  Jiaojiao Li; Mengtong Tang; Hao Qi
Journal:  Front Bioeng Biotechnol       Date:  2022-05-13

4.  A protein inventory of human ribosome biogenesis reveals an essential function of exportin 5 in 60S subunit export.

Authors:  Thomas Wild; Peter Horvath; Emanuel Wyler; Barbara Widmann; Lukas Badertscher; Ivo Zemp; Karol Kozak; Gabor Csucs; Elsebet Lund; Ulrike Kutay
Journal:  PLoS Biol       Date:  2010-10-26       Impact factor: 8.029

Review 5.  Engine out of the chassis: cell-free protein synthesis and its uses.

Authors:  Gabriel Rosenblum; Barry S Cooperman
Journal:  FEBS Lett       Date:  2013-10-22       Impact factor: 4.124

6.  Isotope-based analysis of modified tRNA nucleosides correlates modification density with translational efficiency.

Authors:  Caterina Brandmayr; Mirko Wagner; Tobias Brückl; Daniel Globisch; David Pearson; Andrea Christa Kneuttinger; Veronika Reiter; Antje Hienzsch; Susanne Koch; Ines Thoma; Peter Thumbs; Stylianos Michalakis; Markus Müller; Martin Biel; Thomas Carell
Journal:  Angew Chem Int Ed Engl       Date:  2012-10-04       Impact factor: 15.336

7.  Prokaryotic-style frameshifting in a plant translation system: conservation of an unusual single-tRNA slippage event.

Authors:  Sawsan Napthine; Marijana Vidakovic; Roseanne Girnary; Olivier Namy; Ian Brierley
Journal:  EMBO J       Date:  2003-08-01       Impact factor: 11.598

8.  Kinetics of initiating polypeptide elongation in an IRES-dependent system.

Authors:  Haibo Zhang; Martin Y Ng; Yuanwei Chen; Barry S Cooperman
Journal:  Elife       Date:  2016-06-02       Impact factor: 8.140
  8 in total

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