Literature DB >> 11345537

PCR detection method of Clostridium scindens and C. hiranonis in human fecal samples.

M Kitahara1, M Sakamoto, Y Benno.   

Abstract

Clostridium scindens and C. hiranonis have high bile acid 7alpha-dehydroxylating activity. Direct PCR and nested PCR with the specific primers for C. scindens and C. hiranonis were developed and tested for the detection of them in human fecal samples. Nested PCR detected C. scindens in all samples, but direct PCR detected it in 27 of 34 samples. Regarding C. hiranonis, nested PCR detected it in only 18 of 34 samples although nested PCR has a high level of sensitivity. Nested PCR was a specific and sensitive method for the detection of C. scindens and C. hiranonis in fecal samples.

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Year:  2001        PMID： 11345537     DOI： 10.1111/j.1348-0421.2001.tb02616.x

Source DB:  PubMed          Journal:  Microbiol Immunol        ISSN： 0385-5600            Impact factor:   1.955


  8 in total

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Authors:  Seiko Narushima; Kikuji Itoha; Yukiko Miyamoto; Sang-Hee Park; Keiko Nagata; Kazuo Kuruma; Kiyohisa Uchida
Journal:  Lipids       Date:  2006-09       Impact factor: 1.646

2.  Targeting the PXR-TLR4 signaling pathway to reduce intestinal inflammation in an experimental model of necrotizing enterocolitis.

Authors:  Kezhen Huang; Subhajit Mukherjee; Vera DesMarais; Joseph M Albanese; Ektor Rafti; Andrew Draghi Ii; Leigh A Maher; Kamal M Khanna; Sridhar Mani; Adam P Matson
Journal:  Pediatr Res       Date:  2018-02-21       Impact factor: 3.756

3.  BaiCD gene cluster abundance is negatively correlated with Clostridium difficile infection.

Authors:  Philipp Solbach; Patrick Chhatwal; Sabrina Woltemate; Evelina Tacconelli; Michael Buhl; Markus Gerhard; Christoph K Thoeringer; Maria J G T Vehreschild; Nathalie Jazmati; Jan Rupp; Michael P Manns; Oliver Bachmann; Sebastian Suerbaum
Journal:  PLoS One       Date:  2018-05-08       Impact factor: 3.240

4.  Developmental stages in microbiota, bile acids, and clostridial species in healthy puppies.

Authors:  Amanda B Blake; Annalis Cigarroa; Hannah L Klein; Mohammad R Khattab; Theresa Keating; Patti Van De Coevering; Jonathan A Lidbury; Jörg M Steiner; Jan S Suchodolski
Journal:  J Vet Intern Med       Date:  2020-10-13       Impact factor: 3.333

5.  Complete Genome Sequence of Peptacetobacter (Clostridium) hiranonis Strain DGF055142, Isolated from Dog Feces from Flagstaff, Arizona, USA, 2019.

Authors:  Nathan E Stone; Amalee E Nunnally; Chandler C Roe; Heidie M Hornstra; David M Wagner; Jason W Sahl
Journal:  Microbiol Resour Announc       Date:  2021-03-04

6.  Evaluation of Techniques for Measuring Microbial Hazards in Bathing Waters: A Comparative Study.

Authors:  Christelle Schang; Rebekah Henry; Peter A Kolotelo; Toby Prosser; Nick Crosbie; Trish Grant; Darren Cottam; Peter O'Brien; Scott Coutts; Ana Deletic; David T McCarthy
Journal:  PLoS One       Date:  2016-05-23       Impact factor: 3.240

7.  Omeprazole Minimally Alters the Fecal Microbial Community in Six Cats: A Pilot Study.

Authors:  Sarah M Schmid; Jan S Suchodolski; Josh M Price; M K Tolbert
Journal:  Front Vet Sci       Date:  2018-04-16

8.  Effect of an extruded animal protein-free diet on fecal microbiota of dogs with food-responsive enteropathy.

Authors:  Francesca Bresciani; Yasushi Minamoto; Jan S Suchodolski; Giorgia Galiazzo; Carla G Vecchiato; Carlo Pinna; Giacomo Biagi; Marco Pietra
Journal:  J Vet Intern Med       Date:  2018-10-23       Impact factor: 3.333
  8 in total

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