Literature DB >> 11342141

Selenomethionine incorporation in Saccharomyces cerevisiae RNA polymerase II.

D A Bushnell1, P Cramer, R D Kornberg.   

Abstract

A protocol for the incorporation of SeMet into yeast proteins is described. Incorporation at a level of about 50% suffices for the location of Se sites in an anomalous difference Fourier map of the 0.5 MDa yeast RNA polymerase II. This shows the utility of the approach as an aid in the model-building of large protein complexes.

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Year:  2001        PMID： 11342141     DOI： 10.1016/s0969-2126(00)00554-2

Source DB:  PubMed          Journal:  Structure        ISSN： 0969-2126            Impact factor:   5.006


  10 in total

1.  Production of selenomethionyl-derivatized proteins in baculovirus-infected insect cells.

Authors:  Ciarán N Cronin; Kheng B Lim; Joe Rogers
Journal:  Protein Sci       Date:  2007-07-27       Impact factor: 6.725

2.  Structure of the mediator head module bound to the carboxy-terminal domain of RNA polymerase II.

Authors:  Philip J J Robinson; David A Bushnell; Michael J Trnka; Alma L Burlingame; Roger D Kornberg
Journal:  Proc Natl Acad Sci U S A       Date:  2012-10-15       Impact factor: 11.205

3.  Incorporation of selenomethionine into induced intracytoplasmic membrane proteins of Rhodobacter species.

Authors:  Philip D Laible; Aaron N Hata; Adam E Crawford; Deborah K Hanson
Journal:  J Struct Funct Genomics       Date:  2005

4.  Mutation of high-affinity methionine permease contributes to selenomethionyl protein production in Saccharomyces cerevisiae.

Authors:  Toshihiko Kitajima; Yasunori Chiba; Yoshifumi Jigami
Journal:  Appl Environ Microbiol       Date:  2010-08-06       Impact factor: 4.792

Review 5.  Selenium incorporation using recombinant techniques.

Authors:  Helen Walden
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2010-03-24

6.  Selenomethionine incorporation in proteins expressed in Lactococcus lactis.

Authors:  Ronnie P-A Berntsson; Nur Alia Oktaviani; Fabrizia Fusetti; Andy-Mark W H Thunnissen; Bert Poolman; Dirk-Jan Slotboom
Journal:  Protein Sci       Date:  2009-05       Impact factor: 6.725

7.  Blocking S-adenosylmethionine synthesis in yeast allows selenomethionine incorporation and multiwavelength anomalous dispersion phasing.

Authors:  Michael G Malkowski; Erin Quartley; Alan E Friedman; Julie Babulski; Yoshiko Kon; Jennifer Wolfley; Meriem Said; Joseph R Luft; Eric M Phizicky; George T DeTitta; Elizabeth J Grayhack
Journal:  Proc Natl Acad Sci U S A       Date:  2007-04-10       Impact factor: 11.205

8.  A practical method for efficient and optimal production of Seleno-methionine-labeled recombinant protein complexes in the insect cells.

Authors:  Sabine Wenzel; Tsuyoshi Imasaki; Yuichiro Takagi
Journal:  Protein Sci       Date:  2019-02-04       Impact factor: 6.725

9.  Genome-wide screen of Saccharomyces cerevisiae null allele strains identifies genes involved in selenomethionine resistance.

Authors:  Jessica Bockhorn; Bharvi Balar; Dongming He; Eden Seitomer; Paul R Copeland; Terri Goss Kinzy
Journal:  Proc Natl Acad Sci U S A       Date:  2008-11-11       Impact factor: 11.205

10.  Crystal structure of the dynein motor domain.

Authors:  Andrew P Carter; Carol Cho; Lan Jin; Ronald D Vale
Journal:  Science       Date:  2011-02-17       Impact factor: 47.728
  10 in total

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