S H Oliveira1, N W Lukacs. 1. University of Michigan Medical School, Department of Pathology, Ann Arbor 48109-0602, USA.
Abstract
OBJECTIVE AND DESIGN: In the present study we investigated the effect of SCF and/or IgE on histamine, TNF-alpha and chemokines released from bone marrow-derived mast cells (BMMC) as well as chemokine receptor expression. MATERIAL AND METHODS: BMMC were derived from femoral bone marrow of CBA/J mice. The purity of BMMC was >98% after 3 weeks. BMMC (2.5 x 10(6) cells/well) were incubated in the presence or absence of either SCF, IgE plus DNP or a combination of SCF and IgE for 6 and 18 h. Cell-free supernatants were recovered to measure CC chemokines, TNF-alpha and histamine release utilizing ELISA assays. CC chemokine family receptors were detected by RT-PCR analysis, and confirmed using functional chemotactic assays. RESULTS: Histamine levels were comparable between SCF and IgE stimulated cells, whereas TNF-alpha production was significantly greater after IgE compared to SCF stimulation. SCF and/or IgE-stimulated BMMC released CC chemokines, CCL22 (MDC), CCL17 (TARC) and CCL2 (MCP-1). Increased mRNA expression of CCR1, CCR2, CCR3, and CCR5 was detected in SCF and IgE-stimulated BMMCs. Functional chemotactic assays confirmed the expression data. CONCLUSION: SCF and IgE can up-regulate the expression of chemokines and chemokine receptors on mast cells. Thus, SCF may play a significant role in their activation and inflammation during allergic responses.
OBJECTIVE AND DESIGN: In the present study we investigated the effect of SCF and/or IgE on histamine, TNF-alpha and chemokines released from bone marrow-derived mast cells (BMMC) as well as chemokine receptor expression. MATERIAL AND METHODS: BMMC were derived from femoral bone marrow of CBA/J mice. The purity of BMMC was >98% after 3 weeks. BMMC (2.5 x 10(6) cells/well) were incubated in the presence or absence of either SCF, IgE plus DNP or a combination of SCF and IgE for 6 and 18 h. Cell-free supernatants were recovered to measure CC chemokines, TNF-alpha and histamine release utilizing ELISA assays. CC chemokine family receptors were detected by RT-PCR analysis, and confirmed using functional chemotactic assays. RESULTS:Histamine levels were comparable between SCF and IgE stimulated cells, whereas TNF-alpha production was significantly greater after IgE compared to SCF stimulation. SCF and/or IgE-stimulated BMMC released CC chemokines, CCL22 (MDC), CCL17 (TARC) and CCL2 (MCP-1). Increased mRNA expression of CCR1, CCR2, CCR3, and CCR5 was detected in SCF and IgE-stimulated BMMCs. Functional chemotactic assays confirmed the expression data. CONCLUSION:SCF and IgE can up-regulate the expression of chemokines and chemokine receptors on mast cells. Thus, SCF may play a significant role in their activation and inflammation during allergic responses.
Authors: Sarah J Collington; Jenny Hallgren; James E Pease; Tatiana G Jones; Barrett J Rollins; John Westwick; K Frank Austen; Timothy J Williams; Michael F Gurish; Charlotte L Weller Journal: J Immunol Date: 2010-04-28 Impact factor: 5.422
Authors: Xiang Xu; Dongji Zhang; Natalya Lyubynska; Paul J Wolters; Nigel P Killeen; Peter Baluk; Donald M McDonald; Samuel Hawgood; George H Caughey Journal: Am J Respir Crit Care Med Date: 2005-10-06 Impact factor: 21.405
Authors: Jenny Hallgren; Tatiana G Jones; J Pablo Abonia; Wei Xing; Alison Humbles; K Frank Austen; Michael F Gurish Journal: Proc Natl Acad Sci U S A Date: 2007-12-10 Impact factor: 11.205
Authors: Andrew Rasky; David M Habiel; Susan Morris; Matthew Schaller; Bethany B Moore; Sem Phan; Steven L Kunkel; Martin Phillips; Cory Hogaboam; Nicholas W Lukacs Journal: Am J Physiol Lung Cell Mol Physiol Date: 2019-11-20 Impact factor: 5.464
Authors: Benjamin D Medoff; Edward Seung; Sandra Hong; Seddon Y Thomas; Barry P Sandall; Jeremy S Duffield; Douglas A Kuperman; David J Erle; Andrew D Luster Journal: J Immunol Date: 2009-01-01 Impact factor: 5.422