Literature DB >> 11326009

Rapid pulsed-field gel electrophoresis protocol for subtyping of Campylobacter jejuni.

E M Ribot1, C Fitzgerald, K Kubota, B Swaminathan, T J Barrett.   

Abstract

We developed a rapid pulsed-field gel electrophoresis (PFGE) protocol for subtyping Campylobacter isolates based on the standardized protocols used by PulseNet laboratories for the subtyping of other food-borne bacterial pathogens. Various combinations of buffers, reagents, reaction conditions (e.g., cell suspension concentration, lysis time, lysis temperature, and restriction enzyme concentration), and electrophoretic parameters were evaluated in an effort to devise a protocol that is simple, rapid, and robust. PFGE analysis of Campylobacter isolates can be completed in 24 to 30 h using this protocol, whereas the most widely used current protocols require 3 to 4 days to complete. Comparison of PFGE patterns obtained in six laboratories showed that subtyping results obtained using this protocol are highly reproducible.

Entities:  

Mesh：

Year:  2001        PMID： 11326009      PMCID： PMC88044          DOI： 10.1128/JCM.39.5.1889-1894.2001

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  16 in total

Review 1.  Genotyping of Campylobacter spp.

Authors:  T M Wassenaar; D G Newell
Journal:  Appl Environ Microbiol       Date:  2000-01       Impact factor: 4.792

2.  High-resolution genotyping of Campylobacter coli identifies clones of epidemiologic and evolutionary significance.

Authors:  J Stanley; D Linton; K Sutherland; C Jones; R J Owen
Journal:  J Infect Dis       Date:  1995-10       Impact factor: 5.226

3.  Serotyping and serology studies of campylobacteriosis associated with consumption of raw milk.

Authors:  R L Vogt; A A Little; C M Patton; T J Barrett; L A Orciari
Journal:  J Clin Microbiol       Date:  1984-11       Impact factor: 5.948

4.  Passive hemagglutination technique for serotyping Campylobacter fetus subsp. jejuni on the basis of soluble heat-stable antigens.

Authors:  J L Penner; J N Hennessy
Journal:  J Clin Microbiol       Date:  1980-12       Impact factor: 5.948

5.  Campylobacter enteritis associated with contaminated water.

Authors:  R L Vogt; H E Sours; T Barrett; R A Feldman; R J Dickinson; L Witherell
Journal:  Ann Intern Med       Date:  1982-03       Impact factor: 25.391

6.  Validity of SmaI-defined genotypes of Campylobacter jejuni examined by SalI, KpnI, and BamHI polymorphisms: evidence of identical clones infecting humans, poultry, and cattle.

Authors:  S L On; E M Nielsen; J Engberg; M Madsen
Journal:  Epidemiol Infect       Date:  1998-06       Impact factor: 2.451

7.  Water-borne outbreak of campylobacter gastroenteritis.

Authors:  S R Palmer; P R Gully; J M White; A D Pearson; W G Suckling; D M Jones; J C Rawes; J L Penner
Journal:  Lancet       Date:  1983-02-05       Impact factor: 79.321

8.  Comparison of PFGE, ribotyping and phage-typing in the epidemiological analysis of Campylobacter jejuni serotype HS2 infections.

Authors:  J R Gibson; C Fitzgerald; R J Owen
Journal:  Epidemiol Infect       Date:  1995-10       Impact factor: 2.451

Review 9.  Campylobacter jejuni--an emerging foodborne pathogen.

Authors:  S F Altekruse; N J Stern; P I Fields; D L Swerdlow
Journal:  Emerg Infect Dis       Date:  1999 Jan-Feb       Impact factor: 6.883

Review 10.  Food-related illness and death in the United States.

Authors:  P S Mead; L Slutsker; V Dietz; L F McCaig; J S Bresee; C Shapiro; P M Griffin; R V Tauxe
Journal:  Emerg Infect Dis       Date:  1999 Sep-Oct       Impact factor: 6.883
View more
  110 in total

1.  Evaluation of methods for subtyping Campylobacter jejuni during an outbreak involving a food handler.

Authors:  C Fitzgerald; L O Helsel; M A Nicholson; S J Olsen; D L Swerdlow; R Flahart; J Sexton; P I Fields
Journal:  J Clin Microbiol       Date:  2001-07       Impact factor: 5.948

2.  A recurring outbreak of Shigella sonnei among traditionally observant Jewish children in New York City: the risks of daycare and household transmission.

Authors:  V Garrett; K Bornschlegel; D Lange; V Reddy; L Kornstein; J Kornblum; A Agasan; M Hoekstra; M Layton; J Sobel
Journal:  Epidemiol Infect       Date:  2006-04-20       Impact factor: 2.451

3.  Antimicrobial resistance of Campylobacter isolates from retail meat in the United States between 2002 and 2007.

Authors:  S Zhao; S R Young; E Tong; J W Abbott; N Womack; S L Friedman; P F McDermott
Journal:  Appl Environ Microbiol       Date:  2010-10-22       Impact factor: 4.792

4.  Identification of Campylobacter jejuni multilocus sequence type ST-21 clonal complex by single-nucleotide polymorphism analysis.

Authors:  E L Best; A J Fox; J A Frost; F J Bolton
Journal:  J Clin Microbiol       Date:  2004-06       Impact factor: 5.948

5.  Genetic relatedness and quinolone resistance of Campylobacter jejuni strains isolated in 2002 in Hong Kong.

Authors:  Yiu-Wai Chu; Man-Yu Chu; Kit-Yee Luey; Yin-Wa Ngan; Ka-Lok Tsang; Kai-Man Kam
Journal:  J Clin Microbiol       Date:  2004-07       Impact factor: 5.948

6.  Sequence-based typing of flaB is a more stable screening tool than typing of flaA for monitoring of Campylobacter populations.

Authors:  Alexander Mellmann; Jan Mosters; Edda Bartelt; Peter Roggentin; Andrea Ammon; Alexander W Friedrich; Helge Karch; Dag Harmsen
Journal:  J Clin Microbiol       Date:  2004-10       Impact factor: 5.948

7.  Comprehensive detection and discrimination of Campylobacter species by use of confocal micro-Raman spectroscopy and multilocus sequence typing.

Authors:  Xiaonan Lu; Qian Huang; William G Miller; D Eric Aston; Jie Xu; Feng Xue; Hongwei Zhang; Barbara A Rasco; Shuo Wang; Michael E Konkel
Journal:  J Clin Microbiol       Date:  2012-06-27       Impact factor: 5.948

8.  Molecular typing and resistance analysis of travel-associated Salmonella enterica serotype Typhi.

Authors:  A Tatavarthy; R Sanderson; K Peak; G Scilabro; P Davenhill; A Cannons; P Amuso
Journal:  J Clin Microbiol       Date:  2012-05-30       Impact factor: 5.948

9.  Role of real-time molecular typing in the surveillance of Campylobacter enteritis and comparison of pulsed-field gel electrophoresis profiles from chicken and human isolates.

Authors:  Sophie Michaud; Suzanne Ménard; Robert D Arbeit
Journal:  J Clin Microbiol       Date:  2005-03       Impact factor: 5.948

10.  Analysis of Salmonella enterica serotype Typhi pulsed-field gel electrophoresis patterns associated with international travel.

Authors:  K Kubota; T J Barrett; M L Ackers; P S Brachman; E D Mintz
Journal:  J Clin Microbiol       Date:  2005-03       Impact factor: 5.948
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.