| Literature DB >> 11321571 |
D R Davis1, J B McAlpine, C J Pazoles, M K Talbot, E A Alder, C White, B M Jonas, B E Murray, G M Weinstock, B L Rogers.
Abstract
Using bioinformatics approaches, 34 potential multidrug resistance (MDR) transporter sequences representing 4 different transporter families were identified in the unannotated Enterococcus faecalis database (TIGR). A functional genomics campaign generating single-gene insertional disruptions revealed several genes whose absence confers significant hypersensitivities to known antimicrobials. We constructed specific strains, disrupted in a variety of previously unpublished, putative MDR transporter genes, as tools to improve the success of whole-cell antimicrobial screening and discovery. Each of the potential transporters was inactivated at the gene level and then phenotypically characterized, both with single disruption mutants and with 2-gene mutants built upon a delta norA deleted strain background.Entities:
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Year: 2001 PMID： 11321571
Source DB: PubMed Journal: J Mol Microbiol Biotechnol ISSN： 1464-1801