BACKGROUND: Prostatic tumors are well known to progress to hormonal therapy-resistant terminal states. At this stage, there are no chemotherapeutic agents to affect clinical outcome. An effective cell death inducer for these prostate cells may be a candidate as an attractive antitumor agent. The extracts from S. repens have been used to improve the state of prostatic diseases and we have attempted to identify the effective component from the extract. METHODS: Cell viability was examined in LNCaP cells, an in vitro model for hormonal therapy-resistant prostatic tumor. RESULTS: We found that exposure of the extract from S. repens resulted in cell death of LNCaP cells. We also identified myristoleic acid as one of the cytotoxic components in the extract. The cell death exhibited both apoptotic and necrotic nuclear morphology as determined by Hoechst 33342 staining. Cell death was also partially associated with caspase activation. CONCLUSIONS: It was demonstrated that the extract from S. repens and myristoleic acid induces mixed cell death of apoptosis and necrosis in LNCaP cells. These results suggest that the extract and myristoleic acid may develop attractive new tools for the treatment of prostate cancer.
BACKGROUND:Prostatic tumors are well known to progress to hormonal therapy-resistant terminal states. At this stage, there are no chemotherapeutic agents to affect clinical outcome. An effective cell death inducer for these prostate cells may be a candidate as an attractive antitumor agent. The extracts from S. repens have been used to improve the state of prostatic diseases and we have attempted to identify the effective component from the extract. METHODS: Cell viability was examined in LNCaP cells, an in vitro model for hormonal therapy-resistant prostatic tumor. RESULTS: We found that exposure of the extract from S. repens resulted in cell death of LNCaP cells. We also identified myristoleic acid as one of the cytotoxic components in the extract. The cell death exhibited both apoptotic and necrotic nuclear morphology as determined by Hoechst 33342 staining. Cell death was also partially associated with caspase activation. CONCLUSIONS: It was demonstrated that the extract from S. repens and myristoleic acid induces mixed cell death of apoptosis and necrosis in LNCaP cells. These results suggest that the extract and myristoleic acid may develop attractive new tools for the treatment of prostate cancer.
Authors: Deepak Poudyal; Phuong Mai Le; Tia Davis; Anne B Hofseth; Alena Chumanevich; Alexander A Chumanevich; Michael J Wargovich; Mitzi Nagarkatti; Prakash S Nagarkatti; Anthony Windust; Lorne J Hofseth Journal: Cancer Prev Res (Phila) Date: 2012-01-31
Authors: José A Olalde Rangel; Meyer Magarici; Francis Amendola; Oswaldo del Castillo Journal: Evid Based Complement Alternat Med Date: 2005-12 Impact factor: 2.629
Authors: Youn Kyung Choi; Jung-Il Kang; Jin Won Hyun; Young Sang Koh; Ji-Hoon Kang; Chang-Gu Hyun; Kyung-Sup Yoon; Kwang Sik Lee; Chun Mong Lee; Tae Yang Kim; Eun-Sook Yoo; Hee-Kyoung Kang Journal: Biomol Ther (Seoul) Date: 2021-03-01 Impact factor: 4.634