Literature DB >> 11294394

Use of an aminooxy linker for the functionalization of oligodeoxyribonucleotides.

E Defrancq1, J Lhomme.   

Abstract

We describe the preparation of oligonucleotides containing a 5'-linker bearing an aminooxy group. Use of the trityl protecting group for the aminooxy moiety allows purification of the modified oligonucleotide by reverse phase HPLC and cleavage in mild acidic conditions. Derivatization with an aldehydic reporter group is efficient and rapid.

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Year:  2001        PMID： 11294394     DOI： 10.1016/s0960-894x(01)00108-1

Source DB:  PubMed          Journal:  Bioorg Med Chem Lett        ISSN： 0960-894X            Impact factor:   2.823


  3 in total

1.  A High-Throughput Process for the Solid-Phase Purification of Synthetic DNA Sequences.

Authors:  Andrzej Grajkowski; Jacek Cieślak; Serge L Beaucage
Journal:  Curr Protoc Nucleic Acid Chem       Date:  2017-06-19

2.  Selective formation of covalent protein heterodimers with an unnatural amino acid.

Authors:  Benjamin M Hutchins; Stephanie A Kazane; Karin Staflin; Jane S Forsyth; Brunhilde Felding-Habermann; Vaughn V Smider; Peter G Schultz
Journal:  Chem Biol       Date:  2011-03-25

3.  Permanent or reversible conjugation of 2'-O- or 5'-O-aminooxymethylated nucleosides with functional groups as a convenient and efficient approach to the modification of RNA and DNA sequences.

Authors:  Jacek Cieslak; Andrzej Grajkowski; Cristina Ausín; Alexei Gapeev; Serge L Beaucage
Journal:  Nucleic Acids Res       Date:  2011-11-08       Impact factor: 16.971
  3 in total

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