Literature DB >> 11283105

PCR-hybridization assay for Mycobacterium avium complex: optimization of detection in peripheral blood from humans.

G Ferrario1, A Gori, A Rossi, L Catozzi, C Molteni, G Marchetti, A Bandera, M C Rossi, A D Esposti, F Franzetti.   

Abstract

We evaluated the sensitivity of a DNA amplification test for the detection of Mycobacterium avium in blood samples using different blood components and different DNA extraction methods. M. avium-inoculated blood samples were processed to obtain separate blood components: peripheral blood mononuclear cells (PBMCs), polymorphonuclear cells (PMNCs), and whole-blood sodium dodecyl sulfate (SDS)-lysate pellets. The sensitivity for the detection of the lowest mycobacterial load (1 CFU/ml) was significantly greater (P < 0.01) with DNA extracted from SDS-lysate pellets than with DNA extracted from PBMCs or PMNCs. Subsequently, DNA extraction methods based on guanidine NaOH, and proteinase were compared. The sensitivity of the guanidine-based method was significantly greater (P < 0.01) than those of the others.

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Year:  2001        PMID： 11283105      PMCID： PMC87988          DOI： 10.1128/JCM.39.4.1638-1643.2001

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  27 in total

Review 1.  Mycobacterium avium complex infection in the acquired immunodeficiency syndrome.

Authors:  C R Horsburgh
Journal:  N Engl J Med       Date:  1991-05-09       Impact factor: 91.245

Review 2.  Mycobacterium avium infection and AIDS: a therapeutic dilemma in rapid evolution.

Authors:  J J Ellner; M J Goldberger; D M Parenti
Journal:  J Infect Dis       Date:  1991-06       Impact factor: 5.226

3.  Comparison of improved BACTEC and Lowenstein-Jensen media for culture of mycobacteria from clinical specimens.

Authors:  P Anargyros; D S Astill; I S Lim
Journal:  J Clin Microbiol       Date:  1990-06       Impact factor: 5.948

4.  Mycobacterium avium complex infection in patients with the acquired immunodeficiency syndrome. A clinicopathologic study.

Authors:  J M Wallace; J B Hannah
Journal:  Chest       Date:  1988-05       Impact factor: 9.410

5.  A PCR-colorimetric microwell plate hybridization assay for detection of Mycobacterium tuberculosis and M. avium from culture samples and Ziehl-Neelsen-positive smears.

Authors:  M C Rossi; A Gori; G Zehender; G Marchetti; G Ferrario; C De Maddalena; L Catozzi; A Bandera; A D Esposti; F Franzetti
Journal:  J Clin Microbiol       Date:  2000-05       Impact factor: 5.948

6.  Detection and identification of mycobacteria by amplification of rRNA.

Authors:  B Böddinghaus; T Rogall; T Flohr; H Blöcker; E C Böttger
Journal:  J Clin Microbiol       Date:  1990-08       Impact factor: 5.948

7.  Polymerase chain reaction for detection of Mycobacterium tuberculosis.

Authors:  U Sjöbring; M Mecklenburg; A B Andersen; H Miörner
Journal:  J Clin Microbiol       Date:  1990-10       Impact factor: 5.948

8.  Use of PCR in detection of Mycobacterium avium complex (MAC) bacteremia: sensitivity of the assay and effect of treatment for MAC infection on concentrations of human immunodeficiency virus in plasma.

Authors:  R R MacGregor; K Dreyer; S Herman; P K Hocknell; L Nghiem; V J Tevere; A L Williams
Journal:  J Clin Microbiol       Date:  1999-01       Impact factor: 5.948

9.  Disseminated Mycobacterium avium complex infection: clinical identification and epidemiologic trends.

Authors:  J A Havlik; C R Horsburgh; B Metchock; P P Williams; S A Fann; S E Thompson
Journal:  J Infect Dis       Date:  1992-03       Impact factor: 5.226

10.  Mycobacterium avium complex infections in patients with the acquired immunodeficiency syndrome.

Authors:  C C Hawkins; J W Gold; E Whimbey; T E Kiehn; P Brannon; R Cammarata; A E Brown; D Armstrong
Journal:  Ann Intern Med       Date:  1986-08       Impact factor: 25.391
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  2 in total

1.  The usefulness of PCR assay in diagnosing disseminated mycobacterial infection in AIDS patients.

Authors:  L Gazzola; F Zanini; P Zerbi; F Franzetti; A Gori
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2007-11-13       Impact factor: 3.267

2.  Spoligotyping and Mycobacterium tuberculosis.

Authors:  Andrea Gori; Alessandra Bandera; Giulia Marchetti; Anna Degli Esposti; Lidia Catozzi; Gian Piero Nardi; Lidia Gazzola; Giulio Ferrario; Jan D A van Embden; Dick van Soolingen; Mauro Moroni; Fabio Franzetti
Journal:  Emerg Infect Dis       Date:  2005-08       Impact factor: 6.883
  2 in total

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