Literature DB >> 2121782

Polymerase chain reaction for detection of Mycobacterium tuberculosis.

U Sjöbring1, M Mecklenburg, A B Andersen, H Miörner.   

Abstract

A polymerase chain reaction for the specific detection of mycobacteria belonging to the Mycobacterium tuberculosis complex was developed. Using a single primer pair derived from the nucleotide sequence of protein antigen b of M. tuberculosis, we achieved specific amplification of a 419-base-pair DNA fragment in M. tuberculosis and M. bovis. After DNA was extracted from mycobacteria by using a simple, safe lysis procedure, we detected the 419-base-pair sequence in samples containing few mycobacteria. Preliminary data suggested that this technique could be applied to clinical specimens for early and specific diagnosis of tuberculosis.

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Year:  1990        PMID： 2121782      PMCID： PMC268147          DOI： 10.1128/jcm.28.10.2200-2204.1990

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  18 in total

1.  Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.

Authors:  R K Saiki; S Scharf; F Faloona; K B Mullis; G T Horn; H A Erlich; N Arnheim
Journal:  Science       Date:  1985-12-20       Impact factor: 47.728

2.  Characterization of serologic and cell-mediated reactivity of a 38-kDa antigen isolated from Mycobacterium tuberculosis.

Authors:  G V Kadival; S D Chaparas; D Hussong
Journal:  J Immunol       Date:  1987-10-01       Impact factor: 5.422

3.  Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.

Authors:  R K Saiki; D H Gelfand; S Stoffel; S J Scharf; R Higuchi; G T Horn; K B Mullis; H A Erlich
Journal:  Science       Date:  1988-01-29       Impact factor: 47.728

Review 4.  Nontuberculous mycobacteria and associated diseases.

Authors:  E Wolinsky
Journal:  Am Rev Respir Dis       Date:  1979-01

5.  Tuberculosis as a manifestation of the acquired immunodeficiency syndrome (AIDS).

Authors:  G Sunderam; R J McDonald; T Maniatis; J Oleske; R Kapila; L B Reichman
Journal:  JAMA       Date:  1986-07-18       Impact factor: 56.272

6.  Isolation and purification of deoxyribonucleic acid from mycobacteria.

Authors:  I Baess
Journal:  Acta Pathol Microbiol Scand B Microbiol Immunol       Date:  1974-12

Review 7.  The serodiagnosis of tuberculosis and other mycobacterial diseases by enzyme-linked immunosorbent assay.

Authors:  T M Daniel; S M Debanne
Journal:  Am Rev Respir Dis       Date:  1987-05

8.  Whole chromosomal DNA probes for rapid identification of Mycobacterium tuberculosis and Mycobacterium avium complex.

Authors:  M C Roberts; C McMillan; M B Coyle
Journal:  J Clin Microbiol       Date:  1987-07       Impact factor: 5.948

9.  Tuberculous meningitis.

Authors:  D H Kennedy; R J Fallon
Journal:  JAMA       Date:  1979-01-19       Impact factor: 56.272

10.  Diagnosis of pulmonary tuberculosis by selected-ion monitoring: improved analysis of tuberculostearate in sputum using negative-ion mass spectrometry.

Authors:  L Larsson; G Odham; G Westerdahl; B Olsson
Journal:  J Clin Microbiol       Date:  1987-05       Impact factor: 5.948
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  65 in total

1.  Rapid detection of tuberculous and non-tuberculous mycobacteria by polymerase chain reaction amplification of a 162 bp DNA fragment from antigen 85.

Authors:  M Fauville-Dufaux; B Vanfleteren; L De Wit; J P Vincke; J P Van Vooren; M D Yates; E Serruys; J Content
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1992-09       Impact factor: 3.267

Review 2.  Molecular biology made easy. The polymerase chain reaction.

Authors:  A M Clarke; N P Mapstone; P Quirke
Journal:  Histochem J       Date:  1992-12

3.  Purification of sputum samples through sucrose improves detection of Mycobacterium tuberculosis by polymerase chain reaction.

Authors:  T Victor; R du Toit; P D van Helden
Journal:  J Clin Microbiol       Date:  1992-06       Impact factor: 5.948

4.  Detection and identification of Mycobacterium species isolates by DNA microarray.

Authors:  Masao Fukushima; Kenichi Kakinuma; Hiroshi Hayashi; Hiroko Nagai; Kunihiko Ito; Ryuji Kawaguchi
Journal:  J Clin Microbiol       Date:  2003-06       Impact factor: 5.948

5.  Comparison of an internally controlled, large-volume LightCycler assay for detection of Mycobacterium tuberculosis in clinical samples with the COBAS AMPLICOR assay.

Authors:  Siegfried Burggraf; Udo Reischl; Naeem Malik; Markus Bollwein; Ludmila Naumann; Bernhard Olgemöller
Journal:  J Clin Microbiol       Date:  2005-04       Impact factor: 5.948

6.  Use of PCR in routine diagnosis of treated and untreated pulmonary tuberculosis.

Authors:  K Y Yuen; K S Chan; C M Chan; B S Ho; L K Dai; P Y Chau; M H Ng
Journal:  J Clin Pathol       Date:  1993-04       Impact factor: 3.411

7.  Amplification of residual DNA sequences in sterile bronchoscopes leading to false-positive PCR results.

Authors:  K Kaul; S Luke; C McGurn; N Snowden; C Monti; W A Fry
Journal:  J Clin Microbiol       Date:  1996-08       Impact factor: 5.948

8.  Detection of Mycobacterium tuberculosis by PCR amplification with pan-Mycobacterium primers and hybridization to an M. tuberculosis-specific probe.

Authors:  V J Tevere; P L Hewitt; A Dare; P Hocknell; A Keen; J P Spadoro; K K Young
Journal:  J Clin Microbiol       Date:  1996-04       Impact factor: 5.948

9.  Evaluation of PCR in detection of Mycobacterium tuberculosis from formalin-fixed, paraffin-embedded tissues: comparison of four amplification assays.

Authors:  G Marchetti; A Gori; L Catozzi; L Vago; M Nebuloni; M C Rossi; A D Esposti; A Bandera; F Franzetti
Journal:  J Clin Microbiol       Date:  1998-06       Impact factor: 5.948

10.  Detection of Bacteroides fragilis in clinical specimens by PCR.

Authors:  Y Yamashita; S Kohno; H Koga; K Tomono; M Kaku
Journal:  J Clin Microbiol       Date:  1994-03       Impact factor: 5.948
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