New application of high pressure reversed-phase liquid chromatography in lipids.

High pressure reverse-phase liquid chromatography has been used to separate saturated fatty acids, their methyl esters, polyunsaturated fatty acids, and triglycerides. Rapid separation of fatty acids differing in chain length and number of double bonds has been accomplished. Analysis time was less than 10 min in most cases. The high pressure reverse chromatography resulted in better separations of polyenoic acids than can be accomplished by conventional argentation silicic acid column chromatography. The analyses were carried out on a chemically bonded reverse phase packing, VYDAC reverse phase.