Literature DB >> 11272827

A simple, rapid, and highly efficient gene expression system for multiheme cytochromes c.

K Ozawa1, F Yasukawa, Y Fujiwara, H Akutsu.   

Abstract

The genes of tetraheme cytochrome c3 and hexadecaheme high-molecular-weight cytochrome c from Desulfovibrio vulgaris could be overexpressed as holoproteins in Shewanella oneidensis TSP-C using pUC-type vectors of E. coli. Surprisingly, S. oneidensis was transformed directly by pUC-type vectors through electroporation. The yields of the recombinant proteins in this expression system were much higher than the previously reported ones.

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Year:  2001        PMID： 11272827     DOI： 10.1271/bbb.65.185

Source DB:  PubMed          Journal:  Biosci Biotechnol Biochem        ISSN： 0916-8451            Impact factor:   2.043


  8 in total

1.  pSW2, a Novel Low-Temperature-Inducible Gene Expression Vector Based on a Filamentous Phage of the Deep-Sea Bacterium Shewanella piezotolerans WP3.

Authors:  Xin-Wei Yang; Hua-Hua Jian; Feng-Ping Wang
Journal:  Appl Environ Microbiol       Date:  2015-06-05       Impact factor: 4.792

2.  Mechanism of proton transfer in [FeFe]-hydrogenase from Clostridium pasteurianum.

Authors:  Adam J Cornish; Katrin Gärtner; Hui Yang; John W Peters; Eric L Hegg
Journal:  J Biol Chem       Date:  2011-09-07       Impact factor: 5.157

3.  Laue crystal structure of Shewanella oneidensis cytochrome c nitrite reductase from a high-yield expression system.

Authors:  Matthew Youngblut; Evan T Judd; Vukica Srajer; Bilal Sayyed; Tyler Goelzer; Sean J Elliott; Marius Schmidt; A Andrew Pacheco
Journal:  J Biol Inorg Chem       Date:  2012-03-02       Impact factor: 3.358

4.  Role of the aromatic ring of Tyr43 in tetraheme cytochrome c(3) from Desulfovibrio vulgaris Miyazaki F.

Authors:  Kiyoshi Ozawa; Yuki Takayama; Fumiko Yasukawa; Tomoaki Ohmura; Michael A Cusanovich; Yusuke Tomimoto; Hideaki Ogata; Yoshiki Higuchi; Hideo Akutsu
Journal:  Biophys J       Date:  2003-11       Impact factor: 4.033

5.  Shewanella oneidensis: a new and efficient system for expression and maturation of heterologous [Fe-Fe] hydrogenase from Chlamydomonas reinhardtii.

Authors:  Kateryna Sybirna; Tatiana Antoine; Pia Lindberg; Vincent Fourmond; Marc Rousset; Vincent Méjean; Hervé Bottin
Journal:  BMC Biotechnol       Date:  2008-09-18       Impact factor: 2.563

6.  Characterization of Hydrogen Metabolism in the Multicellular Green Alga Volvox carteri.

Authors:  Adam J Cornish; Robin Green; Katrin Gärtner; Saundra Mason; Eric L Hegg
Journal:  PLoS One       Date:  2015-04-30       Impact factor: 3.240

7.  Roles of charged residues in pH-dependent redox properties of cytochrome c3 from Desulfovibrio vulgaris Miyazaki F.

Authors:  Naoki Yahata; Kiyoshi Ozawa; Yusuke Tomimoto; Kumiko Morita; Hirofumi Komori; Hideaki Ogata; Yoshiki Higuchi; Hideo Akutsu
Journal:  Biophysics (Nagoya-shi)       Date:  2006-07-21

8.  Discovery and characterization of a prevalent human gut bacterial enzyme sufficient for the inactivation of a family of plant toxins.

Authors:  Nitzan Koppel; Jordan E Bisanz; Maria-Eirini Pandelia; Peter J Turnbaugh; Emily P Balskus
Journal:  Elife       Date:  2018-05-15       Impact factor: 8.140
  8 in total

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