Literature DB >> 1126946

Tryptophan hydroxylase. Purification and some properties of the enzyme from rabbit hindbrain.

J H Tong, S Kaufman.   

Abstract

Tryptophan hydroxylase from rabbit hindbrain has been extensively purified. It is estimated that the enzyme is between 85 and 90% pure and has a molecular weight of 230, 000. Sodium dodecyl sulfate gel electrophoresis shows that the enzyme is composed of two subunits very close in molecular weight (57, 500 and 60, 900). The substrate specificity and the reaction stoichiometry catalyzed by the enzyme in the presence of 6, 7-dimethyltetrahydropterin, 6-methyltetrahydropterin, and tetrahydrobiopterin have been determined. The effect of some natural occurring phospholipids on the purified enzyme was investigated.

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Year:  1975        PMID： 1126946

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  14 in total

1.  The control of 5-hydroxytryptamine and dopamine synthesis in the brain: a theoretical approach.

Authors:  F A Hommes; J S Lee
Journal:  J Inherit Metab Dis       Date:  1990       Impact factor: 4.982

2.  Regulation and crystallization of phosphorylated and dephosphorylated forms of truncated dimeric phenylalanine hydroxylase.

Authors:  B Kobe; I G Jennings; C M House; S C Feil; B J Michell; T Tiganis; M W Parker; R G Cotton; B E Kemp
Journal:  Protein Sci       Date:  1997-06       Impact factor: 6.725

3.  Full-length cDNA for rabbit tryptophan hydroxylase: functional domains and evolution of aromatic amino acid hydroxylases.

Authors:  H E Grenett; F D Ledley; L L Reed; S L Woo
Journal:  Proc Natl Acad Sci U S A       Date:  1987-08       Impact factor: 11.205

Review 4.  Structure and function of the aromatic amino acid hydroxylases.

Authors:  S E Hufton; I G Jennings; R G Cotton
Journal:  Biochem J       Date:  1995-10-15       Impact factor: 3.857

5.  Characteristics of the activation by dithiothreitol and Fe(2+) of tryptophan hydroxylase from the rat brain.

Authors:  M Hamon; S Bourgoin; F Héry; G Simonnet
Journal:  Neurochem Res       Date:  1978-10       Impact factor: 3.996

6.  Mechanism of irreversible inactivation of phenylalanine-4- and tryptophan-5-hydroxylases by [4-36Cl, 2-14C]p-chlorophenylalanine: a revision.

Authors:  E M Gál; D H Whitacre
Journal:  Neurochem Res       Date:  1982-01       Impact factor: 3.996

7.  Biopterin. VII. Inhibition of synthesis of reduced biopterins and its bearing on the function of cerebral tryptophan-5-hydroxylase in vivo.

Authors:  E M Gál; D H Whitacre
Journal:  Neurochem Res       Date:  1981-03       Impact factor: 3.996

Review 8.  Advances in the molecular characterization of tryptophan hydroxylase.

Authors:  S M Mockus; K E Vrana
Journal:  J Mol Neurosci       Date:  1998-06       Impact factor: 3.444

9.  High-level expression and deletion mutagenesis of human tryptophan hydroxylase.

Authors:  X J Yang; S Kaufman
Journal:  Proc Natl Acad Sci U S A       Date:  1994-07-05       Impact factor: 11.205

10.  Tetrahydrobiopterin Biosynthesis as a Potential Target of the Kynurenine Pathway Metabolite Xanthurenic Acid.

Authors:  Hirohito Haruki; Ruud Hovius; Miriam Grønlund Pedersen; Kai Johnsson
Journal:  J Biol Chem       Date:  2015-11-12       Impact factor: 5.157
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