Literature DB >> 11266572

Assembly of large genomic segments in artificial chromosomes by homologous recombination in Escherichia coli.

M Sosio1, E Bossi, S Donadio.   

Abstract

We developed a method for the reconstruction of a 100 kb DNA fragment into a bacterial artificial chromosome (BAC). The procedure makes use of iterative rounds of homologous recombination in Escherichia coli. Smaller, overlapping fragments of cloned DNA, such as cosmid clones, are required. They are transferred first into a temperature-sensitive replicon and then into the BAC of choice. We demonstrated the usefulness of this procedure by assembling a 90 kb genomic segment into an E.coli-STREPTOMYCES: artificial chromosome (ESAC). Using this procedure, ESACs are easy to handle and remarkably more stable than the starting cosmids.

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Year:  2001        PMID： 11266572      PMCID： PMC31298          DOI： 10.1093/nar/29.7.e37

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  23 in total

1.  Efficient and precise engineering of a 200 kb beta-globin human/bacterial artificial chromosome in E. coli DH10B using an inducible homologous recombination system.

Authors:  K Narayanan; R Williamson; Y Zhang; A F Stewart; P A Ioannou
Journal:  Gene Ther       Date:  1999-03       Impact factor: 5.250

2.  Rapid modification of bacterial artificial chromosomes by ET-recombination.

Authors:  J P Muyrers; Y Zhang; G Testa; A F Stewart
Journal:  Nucleic Acids Res       Date:  1999-03-15       Impact factor: 16.971

3.  Artificial chromosomes for antibiotic-producing actinomycetes.

Authors:  M Sosio; F Giusino; C Cappellano; E Bossi; A M Puglia; S Donadio
Journal:  Nat Biotechnol       Date:  2000-03       Impact factor: 54.908

4.  Recombinational construction in Escherichia coli of infectious adenoviral genomes.

Authors:  J Crouzet; L Naudin; C Orsini; E Vigne; L Ferrero; A Le Roux; P Benoit; M Latta; C Torrent; D Branellec; P Denèfle; J F Mayaux; M Perricaudet; P Yeh
Journal:  Proc Natl Acad Sci U S A       Date:  1997-02-18       Impact factor: 11.205

Review 5.  Pathogenicity islands: bacterial evolution in quantum leaps.

Authors:  E A Groisman; H Ochman
Journal:  Cell       Date:  1996-11-29       Impact factor: 41.582

6.  Homologous recombination based modification in Escherichia coli and germline transmission in transgenic mice of a bacterial artificial chromosome.

Authors:  X W Yang; P Model; N Heintz
Journal:  Nat Biotechnol       Date:  1997-09       Impact factor: 54.908

7.  recA-independent and recA-dependent intramolecular plasmid recombination. Differential homology requirement and distance effect.

Authors:  X Bi; L F Liu
Journal:  J Mol Biol       Date:  1994-01-14       Impact factor: 5.469

8.  Engineered biosynthesis of a complete macrolactone in a heterologous host.

Authors:  C M Kao; L Katz; C Khosla
Journal:  Science       Date:  1994-07-22       Impact factor: 47.728

9.  Escherichia coli host strains SURE and SRB fail to preserve a palindrome cloned in lambda phage: improved alternate host strains.

Authors:  J P Doherty; R Lindeman; R J Trent; M W Graham; D M Woodcock
Journal:  Gene       Date:  1993-02-14       Impact factor: 3.688

10.  Tris-dependent oxidative DNA strand scission during electrophoresis.

Authors:  T Ray; A Mills; P Dyson
Journal:  Electrophoresis       Date:  1995-06       Impact factor: 3.535
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  6 in total

1.  Biosynthesis of Aurodox, a Type III Secretion System Inhibitor from Streptomyces goldiniensis.

Authors:  Rebecca E McHugh; John T Munnoch; Robyn E Braes; Iain J W McKean; Josephine Giard; Andrea Taladriz-Sender; Frederik Peschke; Glenn A Burley; Andrew J Roe; Paul A Hoskisson
Journal:  Appl Environ Microbiol       Date:  2022-07-18       Impact factor: 5.005

2.  Novel bacterial artificial chromosome vector pUvBBAC for use in studies of the functional genomics of Listeria spp.

Authors:  Torsten Hain; Sonja Otten; Ulrich von Both; Som S Chatterjee; Ulrike Technow; André Billion; Rohit Ghai; Walid Mohamed; Eugen Domann; Trinad Chakraborty
Journal:  Appl Environ Microbiol       Date:  2008-01-25       Impact factor: 4.792

3.  The Saccharomyces cerevisiae SCRaMbLE system and genome minimization.

Authors:  Jessica Dymond; Jef Boeke
Journal:  Bioeng Bugs       Date:  2012-05-01

4.  Rapid creation of BAC-based human artificial chromosome vectors by transposition with synthetic alpha-satellite arrays.

Authors:  Joydeep Basu; Gregory Stromberg; George Compitello; Huntington F Willard; Gil Van Bokkelen
Journal:  Nucleic Acids Res       Date:  2005-01-26       Impact factor: 16.971

Review 5.  Artificial chromosomes to explore and to exploit biosynthetic capabilities of actinomycetes.

Authors:  Rosa Alduina; Giuseppe Gallo
Journal:  J Biomed Biotechnol       Date:  2012-08-07

6.  The soybean-Phytophthora resistance locus Rps1-k encompasses coiled coil-nucleotide binding-leucine rich repeat-like genes and repetitive sequences.

Authors:  Hongyu Gao; Madan K Bhattacharyya
Journal:  BMC Plant Biol       Date:  2008-03-19       Impact factor: 4.215
  6 in total

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