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Literature DB >> 10037821

Rapid modification of bacterial artificial chromosomes by ET-recombination.

J P Muyrers¹, Y Zhang, G Testa, A F Stewart.

Abstract

We present a method to modify bacterial artificial chromosomes (BACs) resident in their host strain. The method is based on homologous recombination by ET-cloning. We have successfully modified BACs at two distinct loci by recombination with a PCR product containing homology arms of 50 nt. The procedure we describe here is rapid, was found to work with high efficiency and should be applicable to any BAC modification desired.

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Year: 1999 PMID： 10037821 PMCID： PMC148353 DOI： 10.1093/nar/27.6.1555

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

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Cited

212 in total

1. An efficient method for high-fidelity BAC/PAC retrofitting with a selectable marker for mammalian cell transfection.

Authors: Z Wang; P Engler; A Longacre; U Storb
Journal: Genome Res Date: 2001-01 Impact factor: 9.043

2. An efficient recombination system for chromosome engineering in Escherichia coli.

Authors: D Yu; H M Ellis; E C Lee; N A Jenkins; N G Copeland; D L Court
Journal: Proc Natl Acad Sci U S A Date: 2000-05-23 Impact factor: 11.205

3. A rapid method for efficient gene replacement in the filamentous fungus Aspergillus nidulans.

Authors: M K Chaveroche; J M Ghigo; C d'Enfert
Journal: Nucleic Acids Res Date: 2000-11-15 Impact factor: 16.971

4. A novel method for constructing gene-targeting vectors.

Authors: K Akiyama; H Watanabe; S Tsukada; H Sasai
Journal: Nucleic Acids Res Date: 2000-08-15 Impact factor: 16.971

5. RecE/RecT and Redalpha/Redbeta initiate double-stranded break repair by specifically interacting with their respective partners.

Authors: J P Muyrers; Y Zhang; F Buchholz; A F Stewart
Journal: Genes Dev Date: 2000-08-01 Impact factor: 11.361

10. Insertion of modifications in the beta-globin locus using GET recombination with single-stranded oligonucleotides and denatured PCR fragments.

Authors: Duangporn Jamsai; Michael Orford; Suthat Fucharoen; Robert Williamson; Panayiotis A Ioannou
Journal: Mol Biotechnol Date: 2003-01 Impact factor: 2.695

Rapid modification of bacterial artificial chromosomes by ET-recombination.

1. An efficient method for high-fidelity BAC/PAC retrofitting with a selectable marker for mammalian cell transfection.

2. An efficient recombination system for chromosome engineering in Escherichia coli.

3. A rapid method for efficient gene replacement in the filamentous fungus Aspergillus nidulans.

4. A novel method for constructing gene-targeting vectors.

5. RecE/RecT and Redalpha/Redbeta initiate double-stranded break repair by specifically interacting with their respective partners.

6. Direct induction of T lymphocyte-specific gene expression by the mammalian Notch signaling pathway.

7. High efficiency mutagenesis, repair, and engineering of chromosomal DNA using single-stranded oligonucleotides.

8. Pivotal role of the non-hr origin of DNA replication in the genesis of defective interfering baculoviruses.

9. Engineering EGFP reporter constructs into a 200 kb human beta-globin BAC clone using GET Recombination.

10. Insertion of modifications in the beta-globin locus using GET recombination with single-stranded oligonucleotides and denatured PCR fragments.