Literature DB >> 11257043

Biochemical characterization of the FEZ-1 metallo-beta-lactamase of Legionella gormanii ATCC 33297T produced in Escherichia coli.

P S Mercuri1, F Bouillenne, L Boschi, J Lamotte-Brasseur, G Amicosante, B Devreese, J van Beeumen, J M Frère, G M Rossolini, M Galleni.   

Abstract

The bla(FEZ-1) gene coding for the metallo-beta-lactamase of Legionella (Fluoribacter) gormanii ATCC 33297T was overexpressed via a T7 expression system in Escherichia coli BL21(DE3)(pLysS). The product was purified to homogeneity in two steps with a yield of 53%. The FEZ-1 metallo-beta-lactamase exhibited a broad-spectrum activity profile, with a preference for cephalosporins such as cephalothin, cefuroxime, and cefotaxime. Monobactams were not hydrolyzed. The beta-lactamase was inhibited by metal chelators. FEZ-1 is a monomeric enzyme with a molecular mass of 29,440 Da which possesses two zinc-binding sites. Its zinc content did not vary in the pH range of 5 to 9, but the presence of zinc ions modified the catalytic efficiency of the enzyme. A model of the FEZ-1 three-dimensional structure was built.

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Year:  2001        PMID： 11257043      PMCID： PMC90452          DOI： 10.1128/AAC.45.4.1254-1262.2001

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.191


  39 in total

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Authors:  S D Scrofani; J Chung; J J Huntley; S J Benkovic; P E Wright; H J Dyson
Journal:  Biochemistry       Date:  1999-11-02       Impact factor: 3.162

2.  Kinetic mechanism of metallo-beta-lactamase L1 from Stenotrophomonas maltophilia.

Authors:  S McManus-Munoz; M W Crowder
Journal:  Biochemistry       Date:  1999-02-02       Impact factor: 3.162

3.  Molecular heterogeneity of the L-1 metallo-beta-lactamase family from Stenotrophomonas maltophilia.

Authors:  F Sanschagrin; J Dufresne; R C Levesque
Journal:  Antimicrob Agents Chemother       Date:  1998-05       Impact factor: 5.191

4.  Zn(II) dependence of the Aeromonas hydrophila AE036 metallo-beta-lactamase activity and stability.

Authors:  M Hernandez Valladares; A Felici; G Weber; H W Adolph; M Zeppezauer; G M Rossolini; G Amicosante; J M Frère; M Galleni
Journal:  Biochemistry       Date:  1997-09-23       Impact factor: 3.162

5.  Characterization and sequence of the Chryseobacterium (Flavobacterium) meningosepticum carbapenemase: a new molecular class B beta-lactamase showing a broad substrate profile.

Authors:  G M Rossolini; N Franceschini; M L Riccio; P S Mercuri; M Perilli; M Galleni; J M Frere; G Amicosante
Journal:  Biochem J       Date:  1998-05-15       Impact factor: 3.857

6.  Crystal structure of the zinc-dependent beta-lactamase from Bacillus cereus at 1.9 A resolution: binuclear active site with features of a mononuclear enzyme.

Authors:  S M Fabiane; M K Sohi; T Wan; D J Payne; J H Bateson; T Mitchell; B J Sutton
Journal:  Biochemistry       Date:  1998-09-08       Impact factor: 3.162

7.  Structure of In31, a blaIMP-containing Pseudomonas aeruginosa integron phyletically related to In5, which carries an unusual array of gene cassettes.

Authors:  N Laraki; M Galleni; I Thamm; M L Riccio; G Amicosante; J M Frère; G M Rossolini
Journal:  Antimicrob Agents Chemother       Date:  1999-04       Impact factor: 5.191

8.  Characterization of VIM-2, a carbapenem-hydrolyzing metallo-beta-lactamase and its plasmid- and integron-borne gene from a Pseudomonas aeruginosa clinical isolate in France.

Authors:  L Poirel; T Naas; D Nicolas; L Collet; S Bellais; J D Cavallo; P Nordmann
Journal:  Antimicrob Agents Chemother       Date:  2000-04       Impact factor: 5.191

9.  The crystal structure of the L1 metallo-beta-lactamase from Stenotrophomonas maltophilia at 1.7 A resolution.

Authors:  J H Ullah; T R Walsh; I A Taylor; D C Emery; C S Verma; S J Gamblin; J Spencer
Journal:  J Mol Biol       Date:  1998-11-20       Impact factor: 5.469

10.  Nucleotide and amino acid sequences of the metallo-beta-lactamase, ImiS, from Aeromonas veronii bv. sobria.

Authors:  T R Walsh; W A Neville; M H Haran; D Tolson; D J Payne; J H Bateson; A P MacGowan; P M Bennett
Journal:  Antimicrob Agents Chemother       Date:  1998-02       Impact factor: 5.191
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  33 in total

1.  Crystal structure of the mobile metallo-β-lactamase AIM-1 from Pseudomonas aeruginosa: insights into antibiotic binding and the role of Gln157.

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Journal:  Antimicrob Agents Chemother       Date:  2012-06-04       Impact factor: 5.191

2.  Biochemical Characterization of CPS-1, a Subclass B3 Metallo-β-Lactamase from a Chryseobacterium piscium Soil Isolate.

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Journal:  Antimicrob Agents Chemother       Date:  2015-12-14       Impact factor: 5.191

3.  Biochemical characterization of the THIN-B metallo-beta-lactamase of Janthinobacterium lividum.

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Journal:  Antimicrob Agents Chemother       Date:  2004-12       Impact factor: 5.191

4.  Three-dimensional structure of the quorum-quenching N-acyl homoserine lactone hydrolase from Bacillus thuringiensis.

Authors:  Dali Liu; Bryan W Lepore; Gregory A Petsko; Pei W Thomas; Everett M Stone; Walter Fast; Dagmar Ringe
Journal:  Proc Natl Acad Sci U S A       Date:  2005-08-08       Impact factor: 11.205

5.  Monitoring the zinc affinity of the metallo-beta-lactamase CphA by automated nanoESI-MS.

Authors:  Kris De Vriendt; Gonzalez Van Driessche; Bart Devreese; Carine Bebrone; Christine Anne; Jean-Marie Frère; Moreno Galleni; Jozef Van Beeumen
Journal:  J Am Soc Mass Spectrom       Date:  2006-01-10       Impact factor: 3.109

6.  Role of zinc content on the catalytic efficiency of B1 metallo beta-lactamases.

Authors:  Matteo Dal Peraro; Alejandro J Vila; Paolo Carloni; Michael L Klein
Journal:  J Am Chem Soc       Date:  2007-02-17       Impact factor: 15.419

7.  Postgenomic scan of metallo-beta-lactamase homologues in rhizobacteria: identification and characterization of BJP-1, a subclass B3 ortholog from Bradyrhizobium japonicum.

Authors:  Magdalena Stoczko; Jean-Marie Frère; Gian Maria Rossolini; Jean-Denis Docquier
Journal:  Antimicrob Agents Chemother       Date:  2006-06       Impact factor: 5.191

Review 8.  Zinc and antibiotic resistance: metallo-beta-lactamases and their synthetic analogues.

Authors:  A Tamilselvi; Govindasamy Mugesh
Journal:  J Biol Inorg Chem       Date:  2008-07-22       Impact factor: 3.358

9.  Structural Insights into Recognition of Hydrolyzed Carbapenems and Inhibitors by Subclass B3 Metallo-β-Lactamase SMB-1.

Authors:  Jun-Ichi Wachino; Yoshihiro Yamaguchi; Shigetarou Mori; Wanchun Jin; Kouji Kimura; Hiromasa Kurosaki; Yoshichika Arakawa
Journal:  Antimicrob Agents Chemother       Date:  2016-06-20       Impact factor: 5.191

Review 10.  Updated functional classification of beta-lactamases.

Authors:  Karen Bush; George A Jacoby
Journal:  Antimicrob Agents Chemother       Date:  2009-12-07       Impact factor: 5.191
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